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Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

DOI :

10.3791/56423-v

January 2nd, 2018

January 2nd, 2018

6,067 Views

1Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, 2Department of Biological Chemistry, David Geffen School of Medicine, 3Molecular Biology Institute Interdepartmental Program, University of California, Los Angeles

We describe a protocol for generating proliferating and quiescent primary human dermal fibroblasts, monitoring transcript decay rates, and identifying differentially decaying genes.

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Decay Rates

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