CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
5.7K Views
•
07:46 min
•
December 11th, 2020
DOI :
December 11th, 2020
•Chapters in this video
0:04
Introduction
0:53
C. elegans Gonad Preparation and Microinjection
1:56
Injected Worm Recovery and Transfer
2:37
PickingC. elegans for Screening
3:17
Single Worm Lysis and PCR
4:22
Restriction Digestion and Agarose Gel Electrophoresis
5:08
Identification of Positively Edited and Homozygous Edited Worms
6:01
Results: Representative C. elegans rbm-3.2 Gene Editing and Screening
7:10
Conclusion
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