April 13th, 2021
•We present the preparation of acute pancreatic tissue slices and their use in confocal laser scanning microscopy to study calcium dynamics simultaneously in a large number of live cells, over long time periods, and with high spatiotemporal resolution.
Tags
Related Videos
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
Real Time In Vivo Tracking of Thymocytes in the Anterior Chamber of the Eye by Laser Scanning Microscopy
Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells
Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples
Imaging of mtHyPer7, a Ratiometric Biosensor for Mitochondrial Peroxide, in Living Yeast Cells
Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope
Biological Samples Preparation for Confocal Microscopy, Image Acquisition Setup, and Confocal Microscopy Imaging
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved