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Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

DOI :

10.3791/62321-v

6:10 min

April 8th, 2021

April 8th, 2021

3,770 Views

1Section of Structural & Synthetic Biology, Department of Infectious Diseases, Faculty of Medicine, Imperial College London, South Kensington Campus

Sample preparation for cryo-electron microscopy (cryo-EM) is a significant bottleneck in the structure determination workflow of this method. Here, we provide detailed methods for using an easy-to-use, three-dimensionally printed block for the preparation of support films to stabilize samples for transmission EM studies.

Tags

Cryo EM Samples

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