JoVE Logo
Faculty Resource Center

Sign In

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

DOI :

10.3791/62321-v

6:10 min

April 8th, 2021

April 8th, 2021

3,671 Views

1Section of Structural & Synthetic Biology, Department of Infectious Diseases, Faculty of Medicine, Imperial College London, South Kensington Campus

Sample preparation for cryo-electron microscopy (cryo-EM) is a significant bottleneck in the structure determination workflow of this method. Here, we provide detailed methods for using an easy-to-use, three-dimensionally printed block for the preparation of support films to stabilize samples for transmission EM studies.

Tags

Cryo EM Samples

-- Views

Related Videos

article

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

article

Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

article

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

article

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

article

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment

article

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

article

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

article

Author Spotlight: Peptidome Extraction from Small Extracellular Vesicles Isolated from Bone Marrow-Derived Macrophages

article

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

article

Author Spotlight: A Novel Method for Comprehensive Cell Component Analysis of Cerebral Blood Clots

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved