July 15th, 2021
•We describe the preparation of ribbons of serial sections and their collection on large transfer support for use as Array Tomography samples, along with automated imaging procedures in a scanning electron microscope. The protocol allows screening, retrieval, and targeted imaging of local, rare events, and the acquisition of large data volumes.
Tags
Related Videos
A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy
Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy
Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)
Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved