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Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

DOI :

10.3791/61847-v

9:47 min

July 15th, 2021

July 15th, 2021

3,741 Views

1Thermo Fisher, 2Universite de Lausanne

We describe the preparation of ribbons of serial sections and their collection on large transfer support for use as Array Tomography samples, along with automated imaging procedures in a scanning electron microscope. The protocol allows screening, retrieval, and targeted imaging of local, rare events, and the acquisition of large data volumes.

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Array Tomography Workflow

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