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Culture Methods to Study Apical-Specific Interactions using Intestinal Organoid Models

DOI :

10.3791/62330-v

7:49 min

March 23rd, 2021

March 23rd, 2021

9,410 Views

1STEMCELL Technologies Ltd., 2STEMCELL Technologies Inc., 3Terry Fox Laboratory, BC Cancer

Here we present two protocols that allow the modeling of intestinal apical-specific interactions. Organoid-derived intestinal monolayers and Air-Liquid Interface (ALI) cultures facilitate the generation of well-differentiated epithelia accessible from both luminal and basolateral sides, whereas polarity-inverted intestinal organoids expose their apical side and are amenable to high throughput assays.

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Culture Methods

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