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Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

DOI :

10.3791/64534-v

October 11th, 2022

October 11th, 2022

2,753 Views

1Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University

The present protocol describes establishing a murine colonic organoid system to study the activity and functioning of colonic stem cells in a claudin-7 knockout model.

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