Direct Stochastic Optical Reconstruction Microscopy Calibration
4:56
Visualization of EV in Three Dimensions
6:16
Post-Acquisition Modification and EV Tracing
7:13
Results: Direct Stochastic Optical Reconstruction Microscopy of Extracellular Vesicles
9:00
Conclusion
Transcript
This protocol employs super-resolution microscopy, specifically direct stochastic optical reconstruction microscopy, also known as dSTORM, to bypass the diffraction limit and to visualize EVs with nanometer precision in three dimensions. One notab
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Direct stochastic optical reconstruction microscopy (dSTORM) is used to bypass the typical diffraction limit of light microscopy and to view exosomes at the nanometer scale. It can be employed in both two and three dimensions to characterize exosomes.