MNase Digestion and Size Selection of Samples for RIBO-seq
6:17
rRNA Depletion and Alkaline Fragmentation of Samples for RNA-seq
7:07
Library Preparation and Size Selection of cDNA Libraries
8:49
Results: Quality of cDNA Libraries and Reads
11:31
Conclusion
Transcript
The ribosome profiling technique, also called RIBO-seq, is currently the most effective tool for studying the process of protein synthesis in vivo. The advantage of this method is its ability to monitor translation by precisely mapping the positio
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Here we describe the stages of sample collection and preparation for RIBO-seq in bacteria. Sequencing of the libraries prepared according to these guidelines results in sufficient data for comprehensive bioinformatic analysis. The protocol we present is simple, uses standard laboratory equipment and takes seven days from lysis to obtaining libraries.