RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
8.1K Views
•
12:05 min
•
August 7th, 2021
DOI :
August 7th, 2021
•Chapters in this video
0:00
Introduciton
0:54
Cell Harvesting and Lysis
4:13
MNase Digestion and Size Selection of Samples for RIBO-seq
6:17
rRNA Depletion and Alkaline Fragmentation of Samples for RNA-seq
7:07
Library Preparation and Size Selection of cDNA Libraries
8:49
Results: Quality of cDNA Libraries and Reads
11:31
Conclusion
Related Videos
13:26
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
10.5K Views
12:57
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
11.4K Views
12:08
Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
4.9K Views
08:34
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
6.6K Views
09:19
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
9.0K Views
06:21
Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq
19.6K Views
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
83.3K Views
12:37
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
38.3K Views
11:22
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
27.7K Views
06:57
Enrichment of mRNA and Bisulfite-mRNA Library Preparation for Next-Generation Sequencing
1.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved