Three-Dimensional In Vitro Biomimetic Model of Neuroblastoma Using Collagen-Based Scaffolds

3.3K Views

•

07:48 min

•

July 9th, 2021

DOI :

10.3791/62627-v

July 9th, 2021

•

Ciara Gallagher*¹^,²^,³, Catherine Murphy*¹^,²^,³, Graeme Kelly⁴, Fergal J. O’Brien³^,⁵^,⁶, Olga Piskareva¹^,²^,³^,⁶^,⁷

¹Cancer Bioengineering Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, ²School of Pharmacy and Biomolecular Sciences, RCSI University of Medicine and Health Sciences, Dublin, Ireland, ³Tissue Engineering Research Group, Department of Anatomy and Regenerative Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, ⁴National Children’s Research Centre, Our Lady's Children's Hospital Crumlin, Dublin, Ireland, ⁵Trinity Centre for Bioengineering, Trinity College Dublin, Dublin, Ireland, ⁶Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland

Summary

Explore More Videos

3D Biomimetic Model

Neuroblastoma

Collagen based Scaffolds

Tumor Microenvironment

Therapeutics

Biomarkers

Patient derived Cells

Cell Attachment

Growth Medium

Cell Proliferation

Experimental Conditions

Batch Consistency

Centrifugation

Chapters in this video

0:04

Introduction

0:45

Neuroblastoma Cell Culture on Scaffolds

2:52

Cell Viability Assessment Within Scaffolds

5:21

Results: Cell Viability and Morphology Assessment of Cells Grown on Scaffolds

7:10

Conclusion

Related Videos

09:13

Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2

8.0K Views

06:14

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

6.0K Views

08:30

Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

12.2K Views

05:54

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

8.5K Views

08:50

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

7.5K Views

08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

7.1K Views

08:15

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

15.4K Views

06:07

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

5.5K Views

06:38

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

5.8K Views

05:20

Zebrafish Model of Neuroblastoma Metastasis

2.7K Views