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A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

DOI :

10.3791/61783-v

January 22nd, 2021

January 22nd, 2021

5,365 Views

1Département de la recherche, Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard, 2UMR-S1113 - IRFAC INSERM, Université de Strasbourg

This protocol presents a novel, robust, and reproducible culture system to generate and grow three-dimensional spheroids from Caco2 colon adenocarcinoma cells. The results provide the first proof-of-concept for the appropriateness of this approach to study cancer stem cell biology, including the response to chemotherapy.

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Three dimensional Model

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