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Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

DOI :

10.3791/56568-v

March 12th, 2018

March 12th, 2018

5,844 Views

1Department of Biomedical Engineering, University of Minnesota - Twin Cities, 2Stem Cell Institute, University of Minnesota - Twin Cities, 3Masonic Cancer Center, University of Minnesota - Twin Cities, 4Lillehei Heart Institute, University of Minnesota - Twin Cities, 5Institute for Engineering in Medicine, University of Minnesota - Twin Cities, 6Department of Biology, Jackson State University

This protocol describes an inverted vertical invasion assay that could be used to quantify the migration and invasion capabilities of cells in a three-dimensional setting while preserving the cell microenvironment. This assay is suitable for rare and/or sensitive cells.

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In Vitro

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