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Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

DOI :

10.3791/59868-v

July 15th, 2019

July 15th, 2019

8,436 Views

1Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine

We present a method for isolating and cultivating primary human salivary gland-derived epithelial cells. These cells exhibit gene expression patterns consistent with them being of salivary epithelial origin and can be grown as salispheres on basement membrane matrices derived from Engelbreth-Holm-Swarm tumor cells or as monolayers on treated culture dishes.

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Isolation

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