Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
5.3K Views
•
10:16 min
•
December 11th, 2021
DOI :
December 11th, 2021
•Artem Eremeev1,3, Lilia Belikova1,2, Evgeny Ruchko1, Egor Volovikov1,5, Olga Zubkova1, Alexy Emelin4, Roman Deev4, Olga Lebedeva1,3, Alexandra Bogomazova1,3, Maria Lagarkova1,3
1Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 4Department of Pathological Anatomy, North-Western State Medical University, 5Department of Biology, Lomonosov State University
Chapters in this video
0:02
Introduction
1:41
Transforming Petri Dishes Into Mini Bioreactors
2:51
Induction of Neuronal Differentiation of iPSCs
3:51
The Formation of Spheroids from Neuroepithelial Precursor Cells
5:38
Obtaining and Cultivation of Organoids
7:59
Results
9:19
Conclusion
Related Videos
07:40
Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders
19.6K Views
10:25
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
21.2K Views
08:30
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
8.7K Views
14:19
2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development
3.8K Views
10:34
Derivation of a Human Brain Organoid with Microglia Development
676 Views
05:40
Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
3.6K Views
01:21
An In Vitro Method to Generate Human Brain Organoids
74 Views
01:25
Generating Induced Pluripotent Stem Cell-Derived Cerebral Organoids
100 Views
01:26
Generating Mature Cerebellar Organoids From Induced Pluripotent Stem Cells Using Single-Use Bioreactors
82 Views
01:27
Generating Forebrain-Type Cerebral Organoids from Human-Induced Pluripotent Stem Cells
57 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved