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Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

DOI :

10.3791/56768-v

10:25 min

January 23rd, 2018

January 23rd, 2018

20,514 Views

1Institute of Reconstructive Neurobiology, University of Bonn, 2Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH)

Cerebral organoids represent a new model system to investigate early human brain development in vitro. This article provides the detailed methodology to efficiently generate homogeneous dorsal forebrain-type organoids from human induced pluripotent stem cells including critical characterization and validation steps.

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