Name: sentinel lymph node mapping and biopsy for endometrial cancer at early stage with laparoscopy
Uploaded: 2021-08-19T14:45:00
Description: Watch this Scientific Journal Video about Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy at JoVE.com

This work was supported in part by grants from the National Natural Science Foundation of China (81772777), Shanghai Science and Technology Commission Medical Guidance Project (18411963700), Clinical Research Plan of SHDC (No. SHDC2020CR4079); Shanghai Pujiang Talents Project (17PJ1401400). We thank the timely help given by Fenghua Ma from radiology department for MR images and Chao Wang from pathology department for pathologic images from Obstetrics and Gynecology Hospital of Fudan University.

All surgery procedures related to the patient described here were approved by the Ethics Committees of Obstetrics &#38; Gynecology Hospital of Fudan University in Shanghai, China. Informed consent was acquired from the patient.
1. Indications for SLN mapping with ICG in EC patients
<ol>
	<li>Ensure that patients are clinically diagnosed with primary EC confined to the uterus with low-risk factors (grade 1 or 2 endometrioid carcinomas, pre-surgical endometrial lesion &#8804;2 cm, and myometri...

<ol>
	<li>Siegel, R. L., Miller, K. D., Jemal, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cancer+statistics,+2019.">Cancer statistics, 2019.</a> CA: A Cancer Journal for Clinicians. 69 (1), 7-34 (2019).</li><li>Bray, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Global+cancer+statistics+2018:+GLOBOCAN+estimates+of+incidence+and+mortality+worldwide+for+36+cancers+in+185+countries.">Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries.</a> CA: A Cancer Journal for Clinicians. 68 (6), 394-424 (2018).</li><li>Morice, P., Leary, A., Creutzberg, C., Abu-Rustum, N., Darai, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometrial+cancer.">Endometrial cancer.</a> Lancet. 387 (10023), 1094-1108 (2016).</li><li>de Boer, S. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adjuvant+chemoradiotherapy+versus+radiotherapy+alone+for+women+with+high-risk+endometrial+cancer+(PORTEC-3):+final+results+of+an+international,+open-label,+multicentre,+randomised,+phase+3+trial.">Adjuvant chemoradiotherapy versus radiotherapy alone for women with high-risk endometrial cancer (PORTEC-3): final results of an international, open-label, multicentre, randomised, phase 3 trial.</a> The Lancet. Oncology. 19 (3), 295-309 (2018).</li><li>Creasman, W. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+pathologic+spread+patterns+of+endometrial+cancer.+A+Gynecologic+Oncology+Group+Study.">Surgical pathologic spread patterns of endometrial cancer. A Gynecologic Oncology Group Study.</a> Cancer. 60 (8), 2035-2041 (1987).</li><li>Bodurtha Smith, A., Fader, A., Tanner, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sentinel+lymph+node+assessment+in+endometrial+cancer:+a+systematic+review+and+meta-analysis.">Sentinel lymph node assessment in endometrial cancer: a systematic review and meta-analysis.</a> American Journal of Obstetrics Gynecology. 216 (5), 459-476 (2017).</li><li>Rossi, E. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+comparison+of+sentinel+lymph+node+biopsy+to+lymphadenectomy+for+endometrial+cancer+staging+(FIRES+trial):+a+multicentre,+prospective,+cohort+study.">A comparison of sentinel lymph node biopsy to lymphadenectomy for endometrial cancer staging (FIRES trial): a multicentre, prospective, cohort study.</a> The Lancet. Oncology. 18 (3), 384-392 (2017).</li><li>Holloway, R. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sentinel+lymph+node+mapping+and+staging+in+endometrial+cancer:+A+Society+of+Gynecologic+Oncology+literature+review+with+consensus+recommendations.">Sentinel lymph node mapping and staging in endometrial cancer: A Society of Gynecologic Oncology literature review with consensus recommendations.</a> Gynecologic Oncology. 146 (2), 405-415 (2017).</li><li>Gezer, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cervical+versus+endometrial+injection+for+sentinel+lymph+node+detection+in+endometrial+cancer:+a+randomized+clinical+trial.">Cervical versus endometrial injection for sentinel lymph node detection in endometrial cancer: a randomized clinical trial.</a> International Journal of Gynecological Cancer. 30 (3), 325-331 (2020).</li><li>Manca, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sentinel+lymph+node+biopsy+in+breast+cancer:+Indications,+contraindications,+and+controversies.">Sentinel lymph node biopsy in breast cancer: Indications, contraindications, and controversies.</a> Clinical Nuclear Medicine. 41 (2), 126-133 (2016).</li><li>Digesu, C. S., Weiss, K. D., Colson, Y. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Near-infrared+sentinel+lymph+node+identification+in+non-small+cell+lung+cancer.">Near-infrared sentinel lymph node identification in non-small cell lung cancer.</a> JAMA Surgery. 153 (5), 487-488 (2018).</li><li>Garau, L. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sentinel+lymph+node+biopsy+in+small+papillary+thyroid+cancer.+A+review+on+novel+surgical+techniques.">Sentinel lymph node biopsy in small papillary thyroid cancer. A review on novel surgical techniques.</a> Endocrine. 62 (2), 340-350 (2018).</li><li>Gonzalez, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sentinel+lymph+node+biopsy:+Past+and+present+implications+for+the+management+of+cutaneous+melanoma+with+nodal+metastasis.">Sentinel lymph node biopsy: Past and present implications for the management of cutaneous melanoma with nodal metastasis.</a> American Journal of Clinical Dermatology. 19, 24-30 (2018).</li><li>Levenback, C. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lymphatic+mapping+and+sentinel+lymph+node+biopsy+in+women+with+squamous+cell+carcinoma+of+the+vulva:+a+gynecologic+oncology+group+study.">Lymphatic mapping and sentinel lymph node biopsy in women with squamous cell carcinoma of the vulva: a gynecologic oncology group study.</a> Journal of Clinical Oncology. 30 (31), 3786-3791 (2012).</li><li>Protic, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prognostic+effect+of+ultra-staging+node-negative+colon+cancer+without+adjuvant+chemotherapy:+A+prospective+national+cancer+institute-sponsored+clinical+trial.">Prognostic effect of ultra-staging node-negative colon cancer without adjuvant chemotherapy: A prospective national cancer institute-sponsored clinical trial.</a> Journal of the American College of Surgeons. 221 (3), 643-651 (2015).</li><li>Price, P. M., Badawi, R. D., Cherry, S. R., Jones, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultra+staging+to+unmask+the+prescribing+of+adjuvant+therapy+in+cancer+patients:+the+future+opportunity+to+image+micrometastases+using+total-body+18F-FDG+PET+scanning.">Ultra staging to unmask the prescribing of adjuvant therapy in cancer patients: the future opportunity to image micrometastases using total-body 18F-FDG PET scanning.</a> The Journal of Nuclear Medicine. 55 (4), 696-697 (2014).</li><li>Concin, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=ESGO/ESTRO/ESP+Guidelines+for+the+management+of+patients+with+endometrial+carcinoma.">ESGO/ESTRO/ESP Guidelines for the management of patients with endometrial carcinoma.</a> Virchows Archiv: An International Journal of Pathology. 478 (2), 153-190 (2021).</li><li>FIGO Committee on Gynecologic Oncology. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=FIGO+staging+for+carcinoma+of+the+vulva,+cervix,+and+corpus+uteri.">FIGO staging for carcinoma of the vulva, cervix, and corpus uteri.</a> International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics. 125 (2), 97-98 (2014).</li><li>Barlin, J. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+importance+of+applying+a+sentinel+lymph+node+mapping+algorithm+in+endometrial+cancer+staging:+beyond+removal+of+blue+nodes.">The importance of applying a sentinel lymph node mapping algorithm in endometrial cancer staging: beyond removal of blue nodes.</a> Gynecologic Oncology. 125 (3), 531-535 (2012).</li><li>Amin, M. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> AJCC Cancer Staging Manual, 8th edition. , (2017).</li><li>Eriksson, A. G. Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Update+on+sentinel+lymph+node+biopsy+in+surgical+staging+of+endometrial+carcinoma.">Update on sentinel lymph node biopsy in surgical staging of endometrial carcinoma.</a> Clinical Medicine. 10 (14), (2021).</li><li>Kelley, M. C., Hansen, N., McMasters, K. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lymphatic+mapping+and+sentinel+lymphadenectomy+for+breast+cancer.">Lymphatic mapping and sentinel lymphadenectomy for breast cancer.</a> The American Journal of Surgery. 188 (1), 49-61 (2004).</li><li>Sakorafas, G. H., Peros, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sentinel+lymph+node+biopsy+in+breast+cancer:+what+a+physician+should+know,+a+decade+after+its+introduction+in+clinical+practice.">Sentinel lymph node biopsy in breast cancer: what a physician should know, a decade after its introduction in clinical practice.</a> European Journal of Cancer Care. 16 (4), 318-321 (2007).</li><li>Niikura, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tracer+injection+sites+and+combinations+for+sentinel+lymph+node+detection+in+patients+with+endometrial+cancer.">Tracer injection sites and combinations for sentinel lymph node detection in patients with endometrial cancer.</a> Gynecologic Oncology. 131 (2), 299-303 (2013).</li><li>Backes, F. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prospective+clinical+trial+of+robotic+sentinel+lymph+node+assessment+with+isosulfane+blue+(ISB)+and+indocyanine+green+(ICG)+in+endometrial+cancer+and+the+impact+of+ultrastaging+(NCT01818739).">Prospective clinical trial of robotic sentinel lymph node assessment with isosulfane blue (ISB) and indocyanine green (ICG) in endometrial cancer and the impact of ultrastaging (NCT01818739).</a> Gynecologic Oncology. 153 (3), 496-499 (2019).</li><li>Holloway, R. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sentinel+lymph+node+mapping+and+staging+in+endometrial+cancer:+A+Society+of+Gynecologic+Oncology+literature+review+with+consensus+recommendations.">Sentinel lymph node mapping and staging in endometrial cancer: A Society of Gynecologic Oncology literature review with consensus recommendations.</a> Gynecologic Oncology. 146 (2), 405-415 (2017).</li><li>Ballester, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection+rate+and+diagnostic+accuracy+of+sentinel-node+biopsy+in+early+stage+endometrial+cancer:+a+prospective+multicentre+study+(SENTI-ENDO).">Detection rate and diagnostic accuracy of sentinel-node biopsy in early stage endometrial cancer: a prospective multicentre study (SENTI-ENDO).</a> The Lancet. Oncology. 12 (5), 469-476 (2011).</li><li>Rossi, E. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+comparison+of+sentinel+lymph+node+biopsy+to+lymphadenectomy+for+endometrial+cancer+staging+(FIRES+trial):+a+multicentre,+prospective,+cohort+study.">A comparison of sentinel lymph node biopsy to lymphadenectomy for endometrial cancer staging (FIRES trial): a multicentre, prospective, cohort study.</a> The Lancet. Oncology. 18 (3), 384-392 (2017).</li><li>Mayoral, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=F-FDG+PET/CT+and+sentinel+lymph+node+biopsy+in+the+staging+of+patients+with+cervical+and+endometrial+cancer.+Role+of+dual-time-point+imaging.">F-FDG PET/CT and sentinel lymph node biopsy in the staging of patients with cervical and endometrial cancer. Role of dual-time-point imaging.</a> Revista Espanola de Medicina Nuclear e Imagen Molecular. 36 (1), 20-26 (2017).</li><li>Lecointre, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diagnostic+accuracy+and+clinical+impact+of+sentinel+lymph+node+sampling+in+endometrial+cancer+at+high+risk+of+recurrence:+A+meta-analysis.">Diagnostic accuracy and clinical impact of sentinel lymph node sampling in endometrial cancer at high risk of recurrence: A meta-analysis.</a> Journal of Clinical Medicine. 9 (12), (2020).</li><li>Bodurtha Smith, A. J., Fader, A. N., Tanner, E. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sentinel+lymph+node+assessment+in+endometrial+cancer:+a+systematic+review+and+meta-analysis.">Sentinel lymph node assessment in endometrial cancer: a systematic review and meta-analysis.</a> American Journal of Obstetrics and Gynecology. 216 (5), 459-476 (2017).</li><li>Bonneau, C., Bricou, A., Barranger, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+position+of+the+sentinel+lymph+node+procedure+in+endometrial+cancer.">Current position of the sentinel lymph node procedure in endometrial cancer.</a> Bulletin du Cancer. 98 (2), 133-145 (2011).</li><li>Tanner, E. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Factors+associated+with+successful+bilateral+sentinel+lymph+node+mapping+in+endometrial+cancer.">Factors associated with successful bilateral sentinel lymph node mapping in endometrial cancer.</a> Gynecologic Oncology. 138 (3), 542-547 (2015).</li><li>Ianieri, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sentinel+lymph+node+biopsy+in+the+treatment+of+endometrial+cancer:+Why+we+fail?+Results+of+a+prospective+multicenter+study+on+the+factors+associated+with+failure+of+node+mapping+with+indocyanine+green.">Sentinel lymph node biopsy in the treatment of endometrial cancer: Why we fail? Results of a prospective multicenter study on the factors associated with failure of node mapping with indocyanine green.</a> Gynecologic and Obstetric Investigation. 84 (4), 383-389 (2019).</li><li>Naoura, I., Canlorbe, G., Bendifallah, S., Ballester, M., Dara&#239;, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relevance+of+sentinel+lymph+node+procedure+for+patients+with+high-risk+endometrial+cancer.">Relevance of sentinel lymph node procedure for patients with high-risk endometrial cancer.</a> Gynecologic Oncology. 136 (1), 60-64 (2015).</li><li>Schiavone, M. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Survival+of+patients+with+uterine+carcinosarcoma+undergoing+sentinel+lymph+node+mapping.">Survival of patients with uterine carcinosarcoma undergoing sentinel lymph node mapping.</a> Annals of Surgical Oncology. 23 (1), 196-202 (2016).</li><li>Soliman, P. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+prospective+validation+study+of+sentinel+lymph+node+mapping+for+high-risk+endometrial+cancer.">A prospective validation study of sentinel lymph node mapping for high-risk endometrial cancer.</a> Gynecologic Oncology. 146 (2), 234-239 (2017).</li></ol>

SLN mapping and biopsy is a more selective and tailored lymph node dissection approach that has been applied in the clinic for nearly 20 years. In the field of EC, SLN mapping and biopsy have been increasingly recommended by several guidelines due to their high diagnostic efficiency in early-stage EC, achieving overall and bilateral detection rates of 89%-95% and 52%-82%, respectively, with a sensitivity of 84%-100% and negative predictive value of 97%-100%22. The present study reports a typical E...

Endometrial cancer (EC) is one of the most common diseases in gynecological oncology, and its incidence is rising1,2. Surgery is the first-line treatment for early-stage EC3,4. The evaluation of lymph node metastasis is an essential part of surgical staging in EC. The Gynecologic Oncology Group (GOG) study 33 demonstrated that lymph node metastases are associated with poor prognosis5.
As a new and essential technique for evaluating lymph node metastasis, sentinel lymph node (SLN) mapping and biopsy have emerged in recent years and have been recommended to be employed for patients with apparent uterine-confined EC according to the National Comprehensive Cancer Network (NCCN) guidelines for uterine cancer6,7,8,9,10. SLN mapping has also been extensively applied in tumors such as breast cancer11, lung cancer12, thyroid cancer13, and melanoma14. Pathologic ultrastaging has achieved good performance in colorectal and gynecological cancer15,16,17 and is recommended by the authoritative European guidelines18. Although principles of SLN mapping for EC staging have been provided in international guidelines10,19, there are no detailed manipulations of surgery in other present works.
This work presents the protocol for detailed SLN mapping and biopsy with ICG in a 28-year-old female who had been clinically diagnosed with early-stage EC, thus, improving and promoting the diagnosis of patients.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>2-mL syringe</td>
			<td>Becton, Dickinson and Company, USA</td>
			<td>301940</td>
			<td></td>
		</tr>
		<tr>
			<td>Coagulation forceps</td>
			<td>Shanghai Medical Instrument (Group) Co., Ltd. Surgical Instrument Factory</td>
			<td>D0A010</td>
			<td></td>
		</tr>
		<tr>
			<td>Fluorescence microscope</td>
			<td>Olympus</td>
			<td>IX73</td>
			<td></td>
		</tr>
		<tr>
			<td>Harmonic scalpel</td>
			<td>Soering GmbH</td>
			<td>SONOCA190</td>
			<td></td>
		</tr>
		<tr>
			<td>Hematoxylin-eosin staining</td>
			<td>Beyotime Biotechnologies Corporation,CN</td>
			<td>C0107</td>
			<td></td>
		</tr>
		<tr>
			<td>Immunohistochemical cytokeratin staining</td>
			<td>MXB Biotechnologies Corporation, CN</td>
			<td>Kit-0020</td>
			<td></td>
		</tr>
		<tr>
			<td>Indocyanine green (ICG)</td>
			<td>Dandong Medical Innovation Pharmaceutical Corporation.</td>
			<td>3599-32-4</td>
			<td></td>
		</tr>
		<tr>
			<td>Pararaffin</td>
			<td>Sangon biotech Co., Ltd.</td>
			<td>A601889</td>
			<td></td>
		</tr>
		<tr>
			<td>Pinpoint Endoscopic Fluorescence Imaging system</td>
			<td>Novadaq Technoloies Bonita, Springs, FL</td>
			<td>NA</td>
			<td></td>
		</tr>
		<tr>
			<td>Propofol Injection</td>
			<td>Fresenius Kabi Deutschland GmbH D-61346 Bad Homburg v.d.H., Germany</td>
			<td>H20030124</td>
			<td></td>
		</tr>
		<tr>
			<td>Silk Braided non-absorbable suture</td>
			<td>Shanghai Ethicon</td>
			<td>SA854G</td>
			<td></td>
		</tr>
		<tr>
			<td>Silk thread</td>
			<td>Beijing AKIN AIEN International Medical Technology Co.,Ltd.</td>
			<td>VCPP71D</td>
			<td></td>
		</tr>
		<tr>
			<td>Suction-Irrigation Tube</td>
			<td>Shanghai SMAF</td>
			<td>YX980D</td>
			<td></td>
		</tr>
		<tr>
			<td>Toothless forceps</td>
			<td>Shanghai Medical Instrument (Group) Co., Ltd. Surgical Instrument Factory</td>
			<td>J41010</td>
			<td></td>
		</tr>
		<tr>
			<td>Trocar</td>
			<td>ETHICON Co,. Ltd, USA</td>
			<td>B10LT, B5LT</td>
			<td></td>
		</tr>
	</tbody>
</table>

sentinel lymph node mapping and biopsy for endometrial cancer at early stage with laparoscopy

Sentinel lymph node (SLN) mapping and biopsy is a promising technique for visualizing and evaluating lymph node status in cancer. This approach has been recommended for low-risk endometrial cancer (EC) patients by authoritative international guidelines, but it has not been performed broadly in China and worldwide. This work aims to describe detailed SLN mapping and biopsy procedures to promote the clinical application. SLN mapping and postoperative pathologic ultrastaging were conducted in a patient with low-risk EC using indocyanine green (ICG) dye to track the SLNs under laparoscopy and resecting them completely for ultrastaging. In conclusion, this protocol describes details of ICG injection, and SLN mapping and biopsy in EC patients based on the experiences gained during clinical practice.

The patient in the present case was a 28-year-old female with irregular vaginal bleeding for 2 years, and she was diagnosed with an abnormality of the endometrium 4 months ago. Transvaginal ultrasound examination revealed a heterogeneous endometrial thickness with an adnexal mass. Abdominopelvic magnetic resonance imaging (MRI) demonstrated a 51 mm x 56 mm x 88 mm mass with a clear boundary within the uterine cavity under a high T2WI&#160;signal in a local hospital. Then, she was evaluated by hysteroscopy, and the endome...

Watch this Scientific Journal Video about Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy at JoVE.com

Sentinel Lymph Node Mapping and Biopsy for Endometrial Cancer at Early Stage with Laparoscopy

This protocol describes the identification and resection of sentinel lymph nodes to make the operation as easy and minimally invasive as possible.

Sentinel lymph node (SLN) mapping and biopsy is a promising technique for visualizing and evaluating lymph node status in cancer. This approach has been ...

Research

JoVE Journal

Cancer Research

The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy

In this study, we injected 1% methylene blue dye (MBD) into the subareolar or peritumoral space of the breast. In the case of breast conserving surgery ...

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

Chromosome mis-segregation leads to aneuploidy, a condition in which cells harbor an imbalanced chromosome number. Several lines of evidence strongly ...

Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry

Bone is a connective tissue constituted of osteoblasts, osteocytes, and osteoclasts and a mineralized extracellular matrix, which gives it its strength ...

Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy

Biopsies are standard of care for cancer treatment and are clinically beneficial as they allow solid tumor diagnosis, prognosis, and personalized ...

An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells

Endometrial cancer is the most common gynecologic malignancy in North America and the incidence is rising worldwide. Treatment consists of surgery with or ...

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Triple-negative breast cancer (TNBC) is an aggressive breast cancer subtype with limited therapeutic options. When compared to patients with less ...

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Tumor-derived paracrine signaling is an overlooked component of local immunosuppression and can lead to a permissive environment for continued cancer ...

Quantification of Tumor Cell Adhesion in Lymph Node Cryosections

Tumor-draining lymph nodes (LNs) are not merely filters of tumor-produced waste. They are one of the most common regional sites of provisional residence ...

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Many cancers are characterized by chromosomal translocations which result in the expression of oncogenic fusion transcription factors. Typically, these ...

Analysis of Lymph Node Volume by Ultra-High-Frequency Ultrasound Imaging in the Braf/Pten Genetically Engineered Mouse Model of Melanoma

Tyr::CreER+,BrafCA/+,Ptenlox/lox&#160;genetically engineered mice (Braf/Pten mice) are widely used as an in vivo model of metastatic melanoma. Once a ...