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Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay

DOI :

10.3791/63137-v

5:15 min

February 10th, 2022

February 10th, 2022

2,948 Views

1Research Group Pharmaceutical Biotechnology, TH Köln - University of Applied Sciences, 2Institute of Technical Chemistry, Leibniz University Hannover

Here, we present a protocol to detect neutralization epitopes on antigen-displaying virus-like particles (VLPs). Immunoprecipitation of the human immunodeficiency virus (HIV)-derived VLPs is performed using envelope glycoproteins-specific monoclonal antibodies coupled to protein G-conjugated magnetic beads. Captured VLPs are subsequently subjected to SDS-PAGE and Western blot-analysis employing viral core protein Gag-specific antibodies.

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Viral Vectors

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