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Optimization of a Quantitative Micro-neutralization Assay

DOI :

10.3791/54897-v

10:09 min

December 14th, 2016

December 14th, 2016

20,167 Views

1Mill Hill Laboratory, The Francis Crick Institute

This study describes an imaging-based micro-neutralization assay to analyze the antigenic relationships between viruses. The protocol employs a flatbed scanner and has four steps, including titration, titration quantitation, neutralization, and neutralization quantitation. The assay works well with current circulating influenza A(H1N1)pdm09, A(H3N2), and B viruses.

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Quantitative Micro neutralization Assay

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