Preparation of the Sucrose Gradient Buffers and PBS Isolation
3:32
Measurement of PBS Fluorescence at 77K
4:00
Results: Spectral Evaluation of Phycobilisomes
5:50
Conclusion
Transcript
It is a simple and a fast protocol that allow people unfamiliar with cyanobacteria to isolate phycobilisome at ease. The advantage of this technique is that it is adapted to a small scale and it is easy to perform. It is a simple, reliable, and ef
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The current protocol details the isolation of phycobilisomes from cyanobacteria by centrifugation through a discontinuous sucrose density gradient. The fractions of intact phycobilisomes are confirmed by 77K fluorescent emission spectrum and SDS-PAGE analysis. The resulting phycobilisome fractions are suitable for negative staining of TEM and mass spectrometry analysis.