JoVE Logo
Faculty Resource Center

Sign In

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

DOI :

10.3791/63459-v

April 6th, 2022

April 6th, 2022

2,069 Views

1Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, 2National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, 3Faculty of Physics, Lomonosov Moscow State University, 4Faculty of Biological and Medical Physics, Moscow Institute of Physics and Technology

Here, we describe a set of methods for characterizing the interaction of proteins with membranes of cells or microvesicles.

-- Views

Related Videos

article

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays

article

A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

article

Quantitative Detection of DNA Protein Crosslinks and Their Post-Translational Modifications

article

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

article

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

article

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

article

Author Spotlight: Efficient Venom Extraction Method from Trichogramma Parasitoid Wasps

article

Author Spotlight: Exploring Mitochondrial Function and Chemical Toxicity Using Drosophila melanogaster

article

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

article

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved