JoVE Logo
Faculty Resource Center

Sign In

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

DOI :

10.3791/54349-v

August 24th, 2016

August 24th, 2016

10,670 Views

1Department of Cellular and Molecular Physiology, Yale University School of Medicine, 2Nanobiology Institute, Yale University, 3Department of Molecular Biophysics and Biochemistry, Yale University, 4Laboratoire de Neurophotonique, Université Paris Descartes, Faculté des Sciences Fondamentales et Biomédicales, Centre National de la Recherche Scientifique (CNRS)

Here, we present a protocol to detect single, SNARE-mediated fusion events between liposomes and supported bilayers in microfluidic channels using polarized TIRFM, with single molecule sensitivity and ~15 msec time resolution. Lipid and soluble cargo release can be detected simultaneously. Liposome size, lipid diffusivity, and fusion pore properties are measured.

-- Views

Related Videos

article

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

article

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

article

In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

article

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

article

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations (Video) | JoVE

article

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry (Video) | JoVE

article

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers (Video) | JoVE

article

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture (Video) | JoVE

article

Three-dimensional Navigation-guided, Prone, Single-position, Lateral Lumbar Interbody Fusion Technique (Video) | JoVE

article

Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved