Microdissection of Tricuspid Valve Leaflet Composite Layers
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Results: Histological Assessment and Biaxial Mechanical Testing
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Conclusion
Transcript
This method can isolate the contributions of tissue layers to the overall tissue behavior, providing new insight into the tissue microstructure. Our microdissection and testing framework allows us to directly compare the mechanical and microstruct
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This protocol describes the biaxial mechanical characterization, polarized spatial frequency domain imaging-based collagen quantification, and microdissection of tricuspid valve leaflets. The provided method elucidates how the leaflet layers contribute to the holistic leaflet behaviors.