Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit

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12:39 min

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January 22nd, 2008

DOI :

January 22nd, 2008

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Linda Doan¹, Edwin S. Monuki¹

¹Department of Developmental and Cell Biology, University of California, Irvine (UCI)

Summary

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Rapid Genotyping

Sigma s Extract N Amp Tissue PCR Kit

PCR Amplification

Electrophoretic Gel

Tissue Preparation

Genotyping Process

Mouse Tail Sample

SYBR Green Extract N Amp Tissue PCR Kit

Real time PCR Amplification

Positive Sample Identification

Prompt Genotype Identification

Chapters in this video

0:00

Title

0:12

Introduction

1:04

Preparing Tissue and PCR Reaction Mixture

6:58

Real Time Quantitative PCR

11:10

Melting Curve Analysis

11:45

Conclusion

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