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Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

2.4K Views

08:03 min

April 6th, 2022

April 6th, 2022

2,400 Views

0:05

Introduction

0:50

Preparation of Vesicle-Free Media

2:02

Salivary Gland Dissection and Extracellular Vesicle Secretion

3:18

Isolation of Extracellular Vesicles

6:02

Results: Analysis of Extracellular Vesicles and Small RNAs

7:22

Conclusion

Transcript

This protocol allows us to use a reduced number of ticks which reduces the cost of colony maintenance and decreases the number of vertebrae animals needed for tick feeding. This protocol requires approximately 20 ticks. However, the main advantage

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The present protocol describes the isolation of microRNAs from tick salivary glands and purified extracellular vesicles. This is a universal procedure that combines commonly used reagents and supplies. The method also allows the use of a small number of ticks, resulting in quality microRNAs that can be readily sequenced.

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