JoVE Logo
Faculty Resource Center

Sign In

Genetic Modification and Recombination of Salivary Gland Organ Cultures

DOI :

10.3791/50060-v

January 28th, 2013

January 28th, 2013

12,668 Views

1Department of Biological Sciences, University at Albany, SUNY

A technique to genetically manipulate epithelial cells within whole ex vivo cultured embryonic mouse submandibular glands (SMGs) using viral gene transfer is described. This method takes advantage of the innate ability of SMG epithelium and mesenchyme to spontaneously recombine after separation and infection of epithelial rudiments with adenoviral vectors.

Tags

Genetic Modification

-- Views

Related Videos

article

Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes

article

PDMS Device Fabrication and Surface Modification

article

Method for Culture of Early Chick Embryos ex vivo (New Culture)

article

Double Whole Mount in situ Hybridization of Early Chick Embryos

article

Preparation of 2-dGuo-Treated Thymus Organ Cultures

article

Isolation of Mouse Salivary Gland Stem Cells

article

DNA Methylation: Bisulphite Modification and Analysis

article

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

article

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

article

DNA Electroporation, Isolation and Imaging of Myofibers

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved