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Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

DOI :

10.3791/56512-v

October 30th, 2017

October 30th, 2017

15,072 Views

1Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 2Department of Biomedical Sciences, GA-PCOM

Here, we present a protocol for the isolation of whole, intact mouse mammary glands to investigate extracellular matrix (ECM) expression and ductal morphology. Mouse #4 abdominal glands were extracted from 8-10 week old female nulliparous mice, fixed in neutral buffered formalin, sectioned and stained using immunohistochemistry for ECM proteins.

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Keywords Mammary Gland

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