Intravital Widefield Fluorescence Microscopy of Pulmonary Microcirculation in Experimental Acute Lung Injury Using a Vacuum-Stabilized Imaging System

2.7K Views

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09:28 min

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April 6th, 2022

DOI :

10.3791/63733-v

April 6th, 2022

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Stefan Hall¹, Sufyan Faridi², Irene Euodia²^,³, Sophie Tanner⁴, Andrew Krzysztof Chojnacki⁵, Kamala D. Patel⁶^,⁷, Juan Zhou²^,⁴, Christian Lehmann¹^,²^,⁴^,⁸

¹Department of Physiology and Biophysics, Dalhousie University, ²Department of Microbiology and Immunology, Dalhousie University, ³Department of Neuroscience, Dalhousie University, ⁴Department of Anesthesia, Pain Management, and Perioperative Medicine, Dalhousie University, ⁵Live Cell Imaging Center, University of Calgary, ⁶Department of Physiology and Pharmacology, University of Calgary, ⁷Department of Biochemistry and Molecular Biology, University of Calgary, ⁸Department of Pharmacology, Dalhousie University

Summary

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Intravital Microscopy

Widefield Fluorescence Microscopy

Pulmonary Microcirculation

Vacuum stabilized System

Vital Signs Monitoring

Chapters in this video

0:04

Introduction

0:36

Preparation of the Vacuum-Stabilized Lung Imaging System

1:10

Intubation

1:57

Ventilation

2:47

Thoracotomy

5:10

Microscopy

6:56

Results: Leukocyte Trafficking and Capillary Perfusion in the Pulmonary Microcirculation

8:48

Conclusion

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