The data presented in this article were obtained as part of a process and quality improvement initiative in collaboration with Prytime Medical to optimize the new partial REBOA technology.

Surgeons are using an FDA-approved REBOA device in trauma patients when it is medically necessary. These data were reviewed by the IRB committee of Grant Medical Center/OhioHealth and were determined to be exempt from human subjects&#39; research. Since no patient information is obtained, written consent from the patients is not required. See the Table of Materials for details about the materials and equipment used in this protocol.
1. Common femoral arterial access
<ol>
	<li>Locate the common femoral artery (CFA) using percutaneous landmarks and ultrasound guidance12.
	<ol>
		<li>Look for one or more of the following signs in the patient: i) systolic blood pressure (SBP) &#60;90 mmHg; ii) transient or non-responder to transfusion; iii) profound refractory shock; iv) out-of-hospital cardiac arrest with return of spontaneous circulation. 
		&#8203;NOTE: Establishing CFA access also allows for quick upsizing if occlusion becomes necessary, reducing the time it takes to achieve aortic occlusion13,14.</li>
	</ol>
	</li>
	<li>For safe arterial access, use the modified Seldinger technique: use a needle to puncture the ventral arterial wall of the CFA at a 45&#176; angle. Insert a compatible guidewire through the needle into the artery and remove the needle. Place the 4 Fr sheath with dilator firmly in place over the wire and into the artery. Remove the wire and dilator, leaving the sheath in place15.</li>
	<li>Transduce the CFA arterial line, verify the waveform, and transduce the pressure to confirm arterial placement.</li>
</ol>
2. REBOA procedure
<ol>
	<li>When REBOA is indicated, have the team prepare the second arterial line. Label one arterial line Proximal and the other arterial line Distal. 
	NOTE: Dual channel arterial lines help guide resuscitation and optimize partial occlusion (Figure 1).
	<ol>
		<li>Identify that REBOA is needed when any of the following conditions are met.
		<ol>
			<li>Look for patients with penetrating or blunt injury who are hypotensive (SBP &#60; 90) and do not respond sufficiently to initial resuscitation with 1 or 2 units of whole blood (or 1:1:1 component therapy) in the trauma bay.</li>
			<li>Look for patients with non-traumatic hemorrhage who are profoundly hypotensive and require blood transfusion to maintain SBP &#62; 90.</li>
			<li>Look for patients who are in arrest but are not beyond salvage according to ATLS guidelines for patients in hypovolemic cardiac arrest1.</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>Using Seldinger technique with an 0.035 inch wire, upsize to a compatible 7 French sheath.</li>
	<li>Prepare the catheter according to the manufacturer&#39;s instructions:
	<ol>
		<li>Using a 30 mL syringe with normal saline, prime the safety valve while leaving the orange peel-away in place. 
		NOTE: It is not necessary to inspect the balloon prior to insertion as all balloons are tested during the manufacturing process. Readvancement of the peel-away over the balloon for insertion is difficult and creates unnecessary, time-consuming steps to reassemble the device.</li>
		<li>Pull the vacuum to evacuate the air and close the stopcock.</li>
		<li>Advance the orange peel-away to straighten and cover atraumatic P-tip for insertion.</li>
		<li>Connect the proximal arterial line to the ART port on the device (Figure 2) and flush. Connect the distal arterial line to the side arm of the sheath.</li>
	</ol>
	</li>
	<li>Perform REBOA
	<ol>
		<li>Insert the orange peel-away into the hemostasis valve on sheath ~5 mm until it stops, and advance the dedicated partial occlusion catheter to the desired aortic zone using the zone markers and measurements on the catheter.</li>
		<li>Remove the orange peel-away from the hemostasis valve.</li>
		<li>Flush both arterial lines after placement of the catheter.</li>
		<li>If available, use imaging, such as x-ray or fluoroscopy, to confirm placement of the balloon prior to inflation. Use radiopaque markers integrated into the catheter on either end of the balloon as references for placement.</li>
		<li>Using the patient&#39;s physiological response as a guide, slowly inflate the balloon with saline using a 30 mL syringe. Inflate to support a proximal systolic blood pressure target of 100-130 mmHg.
		<ol>
			<li>To perform partial REBOA, verify the presence of pulsatile flow on the distal arterial waveform measured from the sheath to ensure partial occlusion. 
			NOTE: Non-pulsatile flow indicates complete aortic occlusion is being performed, even if the SBP is non-zero. Titration of distal pressure is a secondary consideration when performing partial occlusion, with a target SBP of 20-50 mmHg when possible.</li>
			<li>If complete occlusion is required, continue inflating until non-pulsatile flow is observed on the distal arterial line.</li>
		</ol>
		</li>
		<li>Secure the device near the sheath with the securement clip.</li>
	</ol>
	</li>
	<li>Provide definitive hemorrhage control.
	<ol>
		<li>Identify and control the source of hemorrhage using appropriate surgical techniques and imaging when clinically indicated. Use clinical judgment to determine the value and risks of additional imaging. 
		NOTE: Contrast-enhanced imaging is feasible while leaving the balloon at partial occlusion to maintain hemodynamic stability while allowing blood and contrast flow past the balloon16 (shown in Video 1). Imaging is associated with a 47% increase in survival at 24 h and a 65% increase in survival at 28 days, despite longer time to initiate surgical hemostasis17.</li>
		<li>Monitor the patient&#39;s vital signs throughout the procedure. 
		NOTE: If a radial arterial line is obtained in the operating room, the SBP will likely be higher than the integrated central aortic pressure monitoring from the catheter due to pulse pressure amplification18.</li>
		<li>Obtain laboratory measures as clinically indicated, including blood gas analysis. 
		NOTE: Do not draw blood from the arterial port on the catheter as the catheter&#39;s length requires a large flush volume and may clot if not completely flushed after a blood draw.</li>
	</ol>
	</li>
	<li>Remove REBOA.
	<ol>
		<li>Deflate the balloon slowly and monitor the patient&#39;s response. 
		NOTE: Gradual deflation facilitates an improved transition to reperfusion.</li>
		<li>If needed, advance a guidewire through the catheter and leave it in place for additional procedures such as endovascular coils for hemorrhage control. 
		NOTE: A guidewire in the catheter is incompatible with pressure monitoring.</li>
		<li>Deflate the balloon using a 30 mL syringe and pull a strong vacuum to ensure complete evacuation of balloon volume; then close the stopcock. Remove the catheter and begin rotating at the 20 cm mark to wrap the balloon around the catheter shaft so it fits through the sheath more easily.
		<ol>
			<li>Do not use excessive force to remove the catheter through the sheath. If resistance is encountered, readvance the catheter to a safe zone of occlusion and reinflate briefly to redistribute the balloon material. Perform the previous removal step again, ensuring a strong vacuum and frequent twisting upon removal. 
			&#8203;NOTE: This balloon has more surface area than previous balloons due to the flow channels, so the fit through the sheath will be tighter.</li>
		</ol>
		</li>
	</ol>
	</li>
</ol>
3. Post-REBOA sheath management and removal
<ol>
	<li>Remove the sheath as soon as possible following the occlusion procedure. 
	NOTE: If there is no plan for subsequent procedure(s) with the 7 French sheath or an emergent care requirement that must be completed as soon as possible, proceed with removal of the sheath. If it is necessary to leave the sheath in place, the sheath must be managed until it is removed.</li>
	<li>Sheath management
	<ol>
		<li>While the sheath is in place, transduce and infuse it with crystalloid or flush it regularly to minimize the risk of thrombosis.</li>
		<li>Conduct hourly vascular checks to assess bilateral pulses; consider Doppler ultrasound and ankle-brachial index measurements19. 
		NOTE: If the patient is transferred, ensure sheath management is handed over and discuss who will remove the sheath.</li>
	</ol>
	</li>
	<li>Sheath removal
	<ol>
		<li>Assess coagulopathy using conventional coagulation assays such as prothrombin time, or viscoelastic assays, including thrombelastography (TEG) and rotational thrombelastometry (ROTEM)20. 
		NOTE: If the patient is coagulopathic, consider reversing coagulopathy prior to sheath removal or surgical repair of the arteriotomy after sheath removal.</li>
		<li>Check bilateral lower extremity pulses to verify full and equal pulses. 
		NOTE: If diminished pulse is noted, notify the attending trauma surgeon, and consider duplex ultrasound or CT angiogram to determine the best course of action to restore function.</li>
		<li>Remove the sheath and close by direct pressure for 30 min or use a closure device per institution policy and physician preference. Ensure the patient is placed on bedrest with affected leg straight for 6 h following sheath removal.</li>
		<li>Perform regular monitoring of the access site21: visualize the site, assess neurovascular function, and monitor vascular function with distal pulse checks and Doppler ultrasound. Monitor the patient every hour for 4 h, and then every 6 h for the next 24 h. If diminished vascular or neurovascular function is noted during any of these checks, immediately notify the attending trauma surgeon, and consider duplex ultrasound or CT angiogram to determine the best course of action to restore function. 
		NOTE: Consider duplex ultrasound 24 h after sheath removal to proactively assess vascular function after sheath removal.</li>
	</ol>
	</li>
</ol>

<ol>
	<li>Cannon, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Resuscitative+Endovascular+Balloon+Occlusion+of+the+Aorta+(REBOA)+for+hemorrhagic+shock.">Resuscitative Endovascular Balloon Occlusion of the Aorta (REBOA) for hemorrhagic shock.</a> Military Medicine. 183, 55-59 (2018).</li><li>Bulger, E. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+use+of+resuscitative+endovascular+balloon+occlusion+of+the+aorta+(REBOA)+in+civilian+trauma+systems+in+the+USA,+2019:+a+joint+statement+from+the+American+College+of+Surgeons+Committee+on+Trauma,+the+American+College+of+Emergency+Physicians,+the+National+Association+of+Emergency+Medical+Services+Physicians+and+the+National+Association+of+Emergency+Medical+Technicians.">Clinical use of resuscitative endovascular balloon occlusion of the aorta (REBOA) in civilian trauma systems in the USA, 2019: a joint statement from the American College of Surgeons Committee on Trauma, the American College of Emergency Physicians, the National Association of Emergency Medical Services Physicians and the National Association of Emergency Medical Technicians.</a> Trauma Surgery &amp; Acute Care Open. 4 (1), 000376 (2019).</li><li>Moore, L. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prospective+observational+evaluation+of+the+ER-REBOA+catheter+at+6+U.S.+trauma+centers.">Prospective observational evaluation of the ER-REBOA catheter at 6 U.S. trauma centers.</a> Annals of Surgery. 275 (2), 520-526 (2020).</li><li>Kemp, M. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+partial+resuscitative+endovascular+balloon+aortic+occlusion+device+that+can+be+deployed+in+zone+1+for+more+than+2+hours+with+minimal+provider+titration.">A novel partial resuscitative endovascular balloon aortic occlusion device that can be deployed in zone 1 for more than 2 hours with minimal provider titration.</a> Journal of Trauma and Acute Care Surgery. 90 (3), 426-433 (2021).</li><li>Sadeghi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood+pressure+targeting+by+partial+REBOA+is+possible+in+severe+hemorrhagic+shock+in+pigs+and+produces+less+circulatory,+metabolic+and+inflammatory+sequelae+than+total+REBOA.">Blood pressure targeting by partial REBOA is possible in severe hemorrhagic shock in pigs and produces less circulatory, metabolic and inflammatory sequelae than total REBOA.</a> Injury. 49 (12), 2132-2141 (2018).</li><li>Forte, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Validation+of+a+novel+partial+Reboa+device+in+a+swine+hemorrhagic+shock+model:+Fine+tuning+flow+to+optimize+bleeding+control+and+reperfusion+injury.">Validation of a novel partial Reboa device in a swine hemorrhagic shock model: Fine tuning flow to optimize bleeding control and reperfusion injury.</a> Journal of Trauma and Acute Care Surgery. 89 (1), 58-67 (2020).</li><li>Madurska, M. 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J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=How+I+do+it:+Partial+resuscitative+endovascular+balloon+occlusion+of+the+aorta+(P-REBOA).">How I do it: Partial resuscitative endovascular balloon occlusion of the aorta (P-REBOA).</a> Journal of Trauma and Acute Care Surgery. 83 (1), 197-199 (2017).</li><li>Bangalore, S., Bhatt, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Femoral+arterial+access+and+closure.">Femoral arterial access and closure.</a> Circulation. 124 (5), 147-156 (2011).</li><li>Vernamonti, J. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Step+Up'+approach+to+the+application+of+REBOA+technology+in+a+rural+trauma+system.">Step Up' approach to the application of REBOA technology in a rural trauma system.</a> Trauma Surgery &amp; Acute Care Open. 4 (1), 000335 (2019).</li><li>Romagnoli, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Time+to+aortic+occlusion:+It's+all+about+access.">Time to aortic occlusion: It's all about access.</a> Journal of Trauma and Acute Care Surgery. 83 (6), 1161-1164 (2017).</li><li>Stannard, A., Eliason, J. L., Rasmussen, T. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Resuscitative+endovascular+balloon+occlusion+of+the+aorta+(REBOA)+as+an+adjunct+for+hemorrhagic+shock.">Resuscitative endovascular balloon occlusion of the aorta (REBOA) as an adjunct for hemorrhagic shock.</a> Journal of Trauma. 71 (6), 1869-1872 (2011).</li><li>Madurska, M. J., Jansen, J. O., Reva, V. A., Mirghani, M., Morrison, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+compatibility+of+computed+tomography+scanning+and+partial+REBOA:+A+large+animal+pilot+study.">The compatibility of computed tomography scanning and partial REBOA: A large animal pilot study.</a> Journal of Trauma and Acute Care Surgery. 83 (3), 557-561 (2017).</li><li>Otsuka, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Is+resuscitative+endovascular+balloon+occlusion+of+the+aorta+for+computed+tomography+diagnosis+feasible+or+not?+A+Japanese+single-center,+retrospective,+observational+study.">Is resuscitative endovascular balloon occlusion of the aorta for computed tomography diagnosis feasible or not? A Japanese single-center, retrospective, observational study.</a> Journal of Trauma and Acute Care Surgery. 91 (2), 287-294 (2021).</li><li>McEniery, C. M., Cockcroft, J. R., Roman, M. J., Franklin, S. S., Wilkinson, I. 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B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Management+of+trauma-induced+coagulopathy+with+thrombelastography.">Management of trauma-induced coagulopathy with thrombelastography.</a> Critical Care Clinics. 33 (1), 119-134 (2017).</li><li>Romagnoli, A., Brenner, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Principles of REBOA. in Hot Topics in Acute Care Surgery and Trauma. Endovascular Resuscitation and Trauma Management: Bleeding and Haemodynamic Control. , (2019).</li><li>Beyer, C. A., Johnson, M. A., Galante, J. M., DuBose, J. 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CEVS and DGB are employed by Prytime Medical Devices, Inc. MCS and MR are part of the limited market release of pREBOA-PRO and are at the first hospital to use pREBOA-PRO. Neither MCS, MR, nor their hospital receives compensation from Prytime Medical Devices, Inc.

Critical steps 
Partial REBOA is a technique that can balance effective control of emergent hemorrhage with mitigation of distal ischemia and reperfusion injury and reduction of supraphysiologic proximal blood pressure upon occlusion. Prior to the dedicated partial REBOA catheter, partial occlusion could only be performed by skilled users11 and required frequent manipulation of balloon volume to maintain the desired level of partial aortic occlusion. The new balloon occlusion technology has integrated flow channels that when apposed to the wall of the aorta, allow a controlled amount of perfusion past the balloon. Dual-channel pressure-monitoring proximal (integrated into the catheter) and distal (side arm of the sheath) allows for pressure-guided therapy during key phases of resuscitation (Figure 1) and safer, more effective delivery of partial REBOA to optimize distal perfusion.
This catheter is currently in limited market release at seven Level I trauma centers in North America, which has illuminated some critical steps in the implementation of occlusion procedures, several of which occur prior to utilizing the device. The first step is to establish and implement hospital or departmental guidelines and/or protocols for obtaining early arterial access on all hypotensive trauma patients. Central aortic blood pressure provides superior hemodynamic monitoring for patients in shock compared to a blood pressure cuff23, which helps to guide resuscitation and assess the need for intervention more accurately. Arterial access also facilitates quick upsizing to a 7 French sheath if progression to REBOA is indicated13,14. The next step is to implement a guideline that outlines patient selection criteria and indications for use of REBOA. It is important to consider early intervention, as higher SBPs upon initiation are associated with improved outcomes24. Guidelines for post REBOA sheath management and routine access site monitoring should also be implemented. Training for all surgeons and staff should be conducted so that they are familiar with their roles and responsibilities during the REBOA procedure. Since dual arterial lines are necessary for blood pressure monitoring proximal (integrated into the catheter) and distal (integrated in the side arm of the sheath) to the site of occlusion, staff should be trained in the timely preparation of dual arterial lines.
Modifications and troubleshooting of the method 
Modifications from this protocol in certain areas are acceptable if there are standardized guidelines/protocols in place at the institution. For instance, there is some variance in preferred initial sheath size for obtaining arterial access, with most utilizing a 4, 5, or 7 French sheath or an 18 G micropuncture kit. Arterial lines smaller than 18 G are not recommended because they are not compatible with the 0.035 inch guidewire required to upsize to a 7 French sheath if REBOA is indicated13. Arterial line alternatives, such as handheld pressure transducer devices25, may also be considered, particularly in low-resource or austere environments.
Limitations of the method 
Since the dedicated partial REBOA catheter allows for precise control of aortic occlusion, the degree of occlusion should be titrated to support a desired proximal blood pressure in the range of SBP 100-130 mmHg. A limitation of the partial occlusion technique is that some patients will not be able to tolerate partial REBOA and will require complete aortic occlusion to achieve hemodynamic stability. Although this catheter supports complete aortic occlusion, certain benefits of partial occlusion will not be observed in these instances (Table 1). Indicators of distal flow should be verified during occlusion to ensure partial occlusion is being performed.
True partial REBOA 
Partial REBOA is an evolution of existing REBOA technology that addresses the intractable clinical problem of distal ischemia and ischemia-reperfusion injury associated with complete aortic occlusion. A dedicated partial REBOA catheter facilitates partial occlusion with minimal provider titration4. There has been increasing utilization of partial REBOA (73% of 80 cases at the Centers of Excellence) as compared to the AORTA database (3% of the 125 cases with occlusion strategy specified, data as of November 2021). The major changes arising from partial occlusion are improved transition to reperfusion and reduction of distal ischemia, especially when occlusion times &#62;30 min are required to complete definitive hemorrhage control. One major change observed in the initial uses from the surgeons at the Centers of Excellence is that there is a significant increase in Zone 1 occlusion with the dedicated partial REBOA catheter (73% of 80 cases at the centers) compared to the AORTA database (67% of 686 cases from the emergence of the 7 French-compatible devices in 2017 to present). Partial REBOA offers the maximal hemodynamic support benefits of Zone 1 occlusion22, while reducing concerns regarding limited safe occlusion time due to distal ischemia4,5,6,7,8.
Although preclinical benefits of partial occlusion have been demonstrated, objective clinical measurements have yet to be obtained. Subjective, surgeon-reported feedback indicates that partial REBOA facilitates specific benefits that are not observed with complete occlusion, including improved transition to reperfusion, extension of safe occlusion time, reduction of distal ischemia, and reduced proximal hypertension (Table 1). Furthermore, surgeons report observed benefits past 30 min, including reduction of distal ischemia and extension of safe occlusion time in 48% and 50% of cases with &#8805;30 min of occlusion, respectively (Figure 3). Although the observed benefits with a dedicated partial REBOA catheter have yet to be quantified clinically, the limited-market release data indicates the ability to perform partial REBOA represents a significant improvement over previous occlusion capabilities and addresses the clinical need to optimize distal perfusion, especially in cases with prolonged occlusion time.

Resuscitative endovascular balloon occlusion of the aorta (REBOA) devices originated from a military-civilian research and development effort to control exsanguination from non-compressible torso hemorrhage as an alternative approach to open aortic occlusion. With the advent of purpose-built devices and advances in REBOA technology, including guidewire-free catheters and compatibility with 7 Fr sheaths available in the second-generation devices, REBOA has become increasingly common in civilian trauma and acute care settings. As is common with military trauma innovations, REBOA has found use in civilian trauma, highlighting the benefit of military trauma innovation to civilian trauma patients. The use of this technique and purpose-built devices has also found application in non-traumatic bleeding, with reports of use in peripartum hemorrhage, gastrointestinal bleeding, tumor resection, and iatrogenic bleeding.
REBOA involves the retrograde advancement of a balloon catheter through the common femoral artery (CFA) to fully occlude the descending aorta allowing the surgeon the time needed to obtain definitive hemorrhage control. Based on the location of the hemorrhage, the balloon can be inflated in the supradiaphragmatic aortic Zone I, extending from the left subclavian artery to the celiac trunk, or in aortic Zone III, which extends from the lowest renal artery to the aortic bifurcation. The most critical limitation of complete aortic occlusion with this procedure is prolonged occlusion times. Longer occlusion generates progressive ischemic effects on downstream tissues and organs and ischemia-reperfusion injuries. Clinical practice guidelines recommend that occlusion times should not exceed 30-60 min for complete Zone I occlusion1,2, as longer occlusion times are associated with an increased risk of ischemic complications and associated reperfusion sequelae, including rebound hypotension and ischemia-reperfusion injury3.
Partial aortic occlusion, in which low volume aortic flow is permitted distal to occlusion, has been proposed as a technique to alleviate the ischemic consequences of complete occlusion. Preclinical literature indicates that compared to complete occlusion, partial occlusion reduces biomarker evidence of organ injury (e.g., lactate, K+, creatinine, pH), reduces adjunct resuscitation requirements (e.g., norepinephrine, bicarbonate)4,5,6, and increases survival with prolonged occlusion times6. Additionally, preliminary clinical literature has demonstrated the benefits of partial occlusion and the feasibility of implementing partial occlusion in a clinical setting. Specifically, University of Maryland Shock Trauma performed a retrospective review of patients treated with Zone 1 partial or complete REBOA. Compared to complete occlusion, partial occlusion significantly reduced vasoactive support requirements and increased the number of patients discharged home in cases requiring &#62;30 min of occlusion, with a trend toward reduction of organ failure and reduced organ support needs7. This suggests that partial REBOA may help to mitigate ischemia and reperfusion injuries, especially in cases requiring prolonged occlusion times7,8. These benefits may also extend to scenarios that are more prone to requiring longer occlusion times, such as austere environments9 and military trauma field care and en route care10.
Due to the nature of compliant balloon technology, standard endovascular balloon occlusion functions in a binary fashion; a small change in balloon volume triggers a significant change in blood flow around the balloon. The result is that the vessel is either completely occluded and distal flow drops to zero, or it is not occluded and near-normal flow resumes. Although gradual transition is possible with existing catheters in the hands of experienced and well-resourced users11, it is difficult to achieve with this current technology as it requires frequent manipulation to maintain the desired level of partial aortic occlusion. The third-generation pREBOA-PRO catheter is currently FDA-cleared and in limited market release at seven Level I trauma centers in North America. It is the first catheter specifically designed to address the limitations of existing technology by enabling partial occlusion with a unique semicompliant balloon design incorporating flow channels that allow for precise control of aortic occlusion to facilitate the balance between hemorrhage control, hemodynamic stability, and distal perfusion. Additionally, the improved control may allow for a gradual transition to reperfusion, presumably avoiding precipitous changes in hemodynamics that complicate resuscitation efforts. This protocol will focus on procedural considerations for partial REBOA, including patient selection criteria and a comparison of complete and partial aortic occlusion with the pREBOA-PRO catheter (herein referred to as a dedicated partial REBOA catheter) in a simulator. Critical steps associated with optimized clinical outcomes using the dedicated partial REBOA catheter will be highlighted. Additionally, a contrast-enhanced CT scan from a trauma patient showing distal perfusion after 2 h of partial aortic occlusion using the dedicated partial REBOA catheter will be reviewed and representative results from the initial uses are discussed.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>IV Pole</td>
			<td>Any</td>
			<td></td>
			<td>Arterial Line Setup</td>
		</tr>
		<tr>
			<td>Jelly</td>
			<td>Any</td>
			<td></td>
			<td>Ultrasound for Vascular Access</td>
		</tr>
		<tr>
			<td>Micropuncture kit</td>
			<td>Cook</td>
			<td>MPIS-405-SST</td>
			<td>21 G needle for vascular access, 4 Fr x 10 cm arterial sheath</td>
		</tr>
		<tr>
			<td>Non-compliant pressure tubing, 2</td>
			<td>Any</td>
			<td></td>
			<td>Arterial Line Setup</td>
		</tr>
		<tr>
			<td>Normal saline, 2x 500 mL</td>
			<td>Any</td>
			<td></td>
			<td>Arterial Line Setup</td>
		</tr>
		<tr>
			<td>pREBOA-PRO Catheter</td>
			<td>Prytime Medical</td>
			<td>PRP7226PRO</td>
			<td>Partial REBOA Catheter</td>
		</tr>
		<tr>
			<td>Pressure bag, 2</td>
			<td>Any</td>
			<td></td>
			<td>Arterial Line Setup</td>
		</tr>
		<tr>
			<td>Probe cover</td>
			<td>Any</td>
			<td></td>
			<td>Ultrasound for Vascular Access</td>
		</tr>
		<tr>
			<td>Probe for vascular access</td>
			<td>Any</td>
			<td></td>
			<td>Ultrasound for Vascular Access</td>
		</tr>
		<tr>
			<td>REBOA Catheter Convenience Set</td>
			<td>Prytime Medical</td>
			<td>KT1835C (US) 
			KT1835E (EU) 
			KT1835CAN (Can)</td>
			<td>18 G needle, 7 Fr introducer sheath, 4x 10 cc saline syringe, 30 cc syringe, scalpel, 2-0 suture, three quarter drape, catheter securement device</td>
		</tr>
		<tr>
			<td>Transducers with extension lines, 2</td>
			<td>Any</td>
			<td></td>
			<td>Arterial Line Setup</td>
		</tr>
		<tr>
			<td>Ultrasound machine</td>
			<td>Any</td>
			<td></td>
			<td>Ultrasound for Vascular Access</td>
		</tr>
		<tr>
			<td>Vital sign monitor &#8211; dual channel BP capable</td>
			<td>Any</td>
			<td></td>
			<td>Arterial Line Setup, displays blood pressure from ART lines above and below the balloon</td>
		</tr>
	</tbody>
</table>

complete and partial resuscitative endovascular balloon occlusion of the aorta for hemorrhagic shock

Resuscitative endovascular balloon occlusion of the aorta (REBOA) devices grew out of a military-civilian partnership to develop new capabilities for hemorrhage control. With the advent of purpose-built devices, REBOA has become increasingly common in civilian trauma and acute care settings. Currently available REBOA catheters were designed as complete aortic occlusion devices. However, the therapeutic window for complete aortic occlusion is time-limited due to ischemia-reperfusion injury. The partial procedure allows blood flow past the level of occlusion while maintaining targeted proximal pressure, which has been shown to reduce distal ischemia and adjunctive resuscitation requirements in preclinical studies with prolonged occlusion times as compared to traditional complete occlusion.
pREBOA-PRO is the first catheter designed to enable partial and complete aortic occlusion and is currently in limited market release at seven Level I trauma centers in North America. This paper will focus on procedural considerations for REBOA, including patient selection criteria and a comparison of complete and partial aortic occlusion in a simulator, along with highlighting critical steps to improve clinical outcomes. Additionally, this paper reviews a contrast-enhanced CT scan from a trauma patient that shows distal perfusion after 2 h of partial aortic occlusion using this newly designed catheter and discusses representative results from the limited market release to highlight the profound effect of technological innovation on outcomes in vascular emergencies.

Currently, the described dedicated partial REBOA catheter is the only FDA-approved catheter designed specifically to enable partial occlusion and is in limited market release at 7 Level I trauma centers in North America. The data provided are qualitative surgeon impressions from experienced REBOA users, but no quantitative measurements are obtained as part of this effort. Caution is warranted when interpreting these initial results; similar outcomes may not be observed when using alternative REBOA devices to deliver partial occlusion.
The third-generation, dedicated, partial REBOA catheter is designed specifically to enable partial occlusion while maintaining many of the key features of the second-generation, complete-occlusion catheters, including integrated blood pressure monitoring and image-free and wire-free use. Additionally, it provides significantly improved precision control of occlusion to deliver easily titratable partial aortic occlusion and improve the transition to reperfusion (Video 2). One major clinical benefit of the dedicated partial REBOA catheter is the ability to precisely control the extent of aortic occlusion to allow perfusion past the balloon, as seen on the CT angiogram performed after 2 h of partial aortic occlusion (Video 1). On the CT, partial occlusion is being performed during contrast-enhanced imaging. Distal perfusion should be monitored in real time by ensuring that distal pulses are present via the arterial line or other appropriate method.
Although zone 1 provides maximal hemodynamic support22, complete occlusion in zone 1 has been associated with short occlusion times to mitigate physiologic hazards. The data show that partial occlusion has increased the use of zone 1 occlusion (80% compared to 67% reported in the AORTA database from 2017 to the present), and that partial REBOA is associated with specific features compared to complete occlusion. As shown in Table 1, compared to complete occlusion, partial occlusion significantly increases the following observed benefits: improved transition to reperfusion (56.9% vs 0%), extension of safe occlusion time (47.1% vs 5.6%), reduction of distal ischemia (39.2% vs 5.6%), with a trend toward reduction of proximal hypertension (21.6% vs 0%). As expected, the observations surrounding reduced interoperative bleeding and reduced blood use are not different between the two occlusion strategies. It should be noted that there may be differences in baseline patient physiology (e.g., SBP, injury severity) between the two occlusion strategies, as the surgeons report patient intolerance of partial REBOA as the main reason for using complete REBOA exclusively (85.7%, n = 12 of 14 respondents).
Additionally, in cases requiring extended (&#8805;30 min) occlusion time, surgeons have observed reduction of distal ischemia (47.5% vs 8.7%) and extension of safe occlusion time (50.0% vs 21.7%) at significantly higher rates than when occlusion times are &#60;30 min (Figure 3). There were six cases where occlusion time was not reported and were eliminated from this analysis. One limitation of these initial observed benefits is the use of subjective observations based on clinical experience and expertise of the surgeons, as these items are not quantitatively measured. Quantification of physiological responses to partial occlusion in the clinical setting would benefit the field.
In summary, partial REBOA significantly increases the observation of improved transition to reperfusion, extension of safe occlusion time, and reduction of distal ischemia compared to complete occlusion (Table 1). Additionally, surgeons report reduction of distal ischemia and extension of safe occlusion time significantly more often in cases requiring &#8805;30 min of occlusion compared to cases of shorter occlusion length (Figure 3). These benefits are associated with partial REBOA, which is characterized by blood flow distal to aortic occlusion (Video 1).
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/63767/63767fig01.jpg" /> 
Figure 1: Use of dual arterial blood pressure monitoring to guide partial REBOA. (A) Partial REBOA is performed using the flow channels incorporated into the semicompliant balloon. Integrated central aortic pressure monitoring from the tip of the catheter is used to guide resuscitation and assess the patient&#39;s response to partial occlusion, providing a blood pressure reading above the site of occlusion. (B) Arterial pressure measured from the CFA sheath is used to assess distal perfusion; presence of pulsatile arterial flow indicates that partial occlusion is being performed. (C) The catheter is placed through the CFA sheath for a singular access site with dual-channel pressure-monitoring capabilities. Abbreviations: REBOA = Resuscitative endovascular balloon occlusion of the aorta; CFA = common femoral artery. <a href="https://www.jove.com/files/ftp_upload/63767/63767fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/63767/63767fig02v2.jpg" /> 
Figure 2: Diagram of pREBOA-PRO catheter and its features. The catheter is characterized by (A) a semicompliant prune balloon that forms flow channels when apposed to the aortic wall, enabling partial REBOA. The catheter includes two extension lines: (B) BAL for balloon inflation with (C) an integrated safety valve to prevent overinflation of the balloon and (D) ART with an integrated arterial line to measure central aortic pressure. Abbreviation: REBOA = Resuscitative endovascular balloon occlusion of the aorta. <a href="https://www.jove.com/files/ftp_upload/63767/63767fig02largev2.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/63767/63767fig03.jpg" /> 
Figure 3: Perfusion-related benefits are observed more frequently in pREBOA-PRO cases requiring extended occlusion times (&#8805;30 min, n = 40) compared to shorter occlusion (&#60;30 min, n = 23). In cases with occlusion times greater than 30 min, reduced distal ischemia and extension of safe occlusion time are observed significantly more frequently than in cases with shorter occlusion times. * indicates p &#60; 0.05, ** indicates p &#60; 0.01 via Fisher&#39;s exact test. Data represent mean &#177; SEM. <a href="https://www.jove.com/files/ftp_upload/63767/63767fig03large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
Video 1: Partial REBOA observed on CT angiogram with perfusion distal to aortic occlusion after 2 h of partial REBOA in zone 1. The patient was in a motor vehicle collision and was persistently hypotensive upon presentation to the trauma bay with a positive FAST exam, suggestive of abdominal hemorrhage. Partial REBOA was deployed in zone 1, and the patient was taken to the operating room for exploratory laparotomy. As no significant sources of bleeding were identified, a full-body contrast-enhanced CT scan was performed after 2 h of partial aortic occlusion. Partial occlusion was performed during the scan, which allowed contrast to pass, while maintaining hemodynamic stability. Abbreviations: REBOA = Resuscitative endovascular balloon occlusion of the aorta; CT = computed tomography; FAST = focused assessment with sonography in trauma. <a href="https://www.jove.com/files/ftp_upload/63767/63767_R3_video 1.mp4" target="_blank">Please click here to download this Video.</a>
Video 2: Side-by-side comparison of REBOA balloon deflation demonstrating improved transition to reperfusion. Both REBOA balloons are inflated to complete occlusion in a silicone tube with 19 mm inner diameter, simulating zone 1 aortic occlusion. Complete aortic occlusion, evidenced by a non-pulsatile waveform from the distal blood pressure, can be observed on the monitors. The balloons are deflated simultaneously at 0.2 cc/s using a syringe puller. The ER-REBOA catheter (left) has a transition volume of 2.3 cc, while pREBOA-PRO (right) has a transition volume of 9.4 cc. The increased transition volume of the dedicated partial occlusion device provides increased control of aortic occlusion and reperfusion. Abbreviation: REBOA = Resuscitative endovascular balloon occlusion of the aorta. <a href="https://www.jove.com/files/ftp_upload/63767/63767_R3_Video2.mp4" target="_blank">Please click here to download this Video.</a>
<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td colspan="2">Observed benefit:</td>
			<td>Partial REBOA N=51 % (n)</td>
			<td>Complete REBOA N=18 % (n)</td>
			<td>p value</td>
		</tr>
		<tr>
			<td colspan="2">Improved transition to reperfusion</td>
			<td>56.9 (29)</td>
			<td>0 (0)</td>
			<td>*p=0.00001</td>
		</tr>
		<tr>
			<td colspan="2">Extension of safe occlusion time</td>
			<td>47.1 (24)</td>
			<td>5.6 (1)</td>
			<td>*p=0.001</td>
		</tr>
		<tr>
			<td colspan="2">Reduced distal ischemia</td>
			<td>39.2 (20)</td>
			<td>5.6 (1)</td>
			<td>*p=0.007</td>
		</tr>
		<tr>
			<td colspan="2">Reduced proximal hypertension</td>
			<td>21.6 (11)</td>
			<td>0 (0)</td>
			<td>+p=0.05</td>
		</tr>
		<tr>
			<td colspan="2">Reduced interoperative bleeding</td>
			<td>45.1 (23)</td>
			<td>22.2 (4)</td>
			<td>p=0.10</td>
		</tr>
		<tr>
			<td colspan="2">Reduced blood use</td>
			<td>33.3 (17)</td>
			<td>11.1 (2)</td>
			<td>p=0.12</td>
		</tr>
	</tbody>
</table>
Table 1: Observed benefits in cases utilizing partial REBOA at any point during the case compared to cases utilizing complete aortic occlusion only. Fisher&#39;s exact test was used to determine whether response rates for observed benefits differed by aortic occlusion strategy (partial or complete). Exact p values are reported, with * indicating p &#60; 0.05 and + indicating p = 0.05.

Watch this Scientific Journal Video about Complete and Partial Resuscitative Endovascular Balloon Occlusion of the Aorta for Hemorrhagic Shock at JoVE.com

Complete and Partial Resuscitative Endovascular Balloon Occlusion of the Aorta for Hemorrhagic Shock

A commercial catheter was designed to facilitate true partial resuscitative endovascular balloon occlusion of the aorta (REBOA) and address the complications associated with complete aortic occlusion. Initial clinical reports indicate that partial REBOA improves transition to reperfusion, reduction of distal ischemia, and extension of safe occlusion time compared to complete occlusion.

Resuscitative endovascular balloon occlusion of the aorta (REBOA) devices grew out of a military-civilian partnership to develop new capabilities for ...

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5.3K Views.  Prytime Medical Device, Inc.. A commercial catheter was designed to facilitate true partial resuscitative endovascular balloon occlusion of the aorta (REBOA) and address the complications associated with complete aortic occlusion. Initial clinical reports indicate that partial REBOA improves transition to reperfusion, reduction of distal ischemia, and extension of safe occlusion time compared to complete occlusion.

Video: Complete and Partial Resuscitative Endovascular Balloon Occlusion of the Aorta for Hemorrhagic Shock

Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock

It is common knowledge that severe blood loss and traumatic injury can lead to a cascade of detrimental signaling events often resulting in mortality. 1, 2, 3, 4, 5 These signaling events can also lead to sepsis and/or multiple organ dysfunction (MOD). 6, 7, 8, 9 It is critical then to investigate the causes of suppressed immune function and detrimental signaling cascades in order to develop more effective ways to help patients who suffer from traumatic injuries. 10 This fixed pressure Hemorrhagic Shock (HS) procedure, although technically challenging, is an excellent resource for investigation of these pathophysiologic conditions. 11, 12, 13 Advances in the assessment of biological systems, i.e. Systems Biology have enabled the scientific community to further understand complex physiologic networks and cellular communication patterns. 14 Hemorrhagic Shock has proven to be a vital tool for unveiling these cellular communication patterns as they relate to immune function. 15, 16, 17, 18 This procedure can be mastered! This procedure can also be used as either a fixed volume or fixed pressure approach. We adapted this technique in the murine model to enhance research in innate and adaptive immune function. 19, 20, 21 Due to their small size HS in mice presents unique challenges. However due to the many available mouse strains, this species represents an unparalleled resource for the study of the biologic responses. The HS model is an important model for studying cellular communication patterns and the responses of systems such as hormonal and inflammatory mediator systems, and danger signals, i.e. DAMP and PAMP upregulation as it elicits distinct responses that differ from other forms of shock. 22, 23, 24, 25 The development of transgenic murine strains and the induction of biologic agents to inhibit specific signaling have presented valuable opportunities to further elucidate our understanding of the up and down regulation of signal transduction after severe blood loss, i.e. HS and trauma 26, 27, 28, 29, 30. 
There are numerous resuscitation methods (R) in association with HS and trauma. 31, 32, 33, 34 A fixed volume resuscitation method of solely lactated ringer solution (LR), equal to three times the shed blood volume, is used in this model to study endogenous mechanisms such as remote organ injury and systemic inflammation. 35, 36, 38 This method of resuscitation is proven to be effective in evaluating the effects of HS and trauma 38, 39.

The Hemorrhagic Shock model has been a reliable and reproducible resource facilitating the identification and understanding of signaling cascades associated with inflammation and end-organ damage after trauma. This article provides a step-by-step description of surgical and mechanical aspects associated with the Hemorrhagic Shock experimental procedure in mice.

It is common knowledge that severe blood loss and traumatic injury can lead to a cascade of detrimental signaling events often resulting in mortality. 1, ...

Permanent Cerebral Vessel Occlusion via Double Ligature and Transection

Stroke is a leading cause of death, disability, and socioeconomic loss worldwide. The majority of all strokes result from an interruption in blood flow (ischemia) 1. Middle cerebral artery (MCA) delivers a great majority of blood to the lateral surface of the cortex 2, is the most common site of human stroke 3, and ischemia within its territory can result in extensive dysfunction or death 1,4,5. Survivors of ischemic stroke often suffer loss or disruption of motor capabilities, sensory deficits, and infarct. In an effort to capture these key characteristics of stroke, and thereby develop effective treatment, a great deal of emphasis is placed upon animal models of ischemia in MCA.
Here we present a method of permanently occluding a cortical surface blood vessel. We will present this method using an example of a relevant vessel occlusion that models the most common type, location, and outcome of human stroke, permanent middle cerebral artery occlusion (pMCAO). In this model, we surgically expose MCA in the adult rat and subsequently occlude via double ligature and transection of the vessel. This pMCAO blocks the proximal cortical branch of MCA, causing ischemia in all of MCA cortical territory, a large portion of the cortex. This method of occlusion can also be used to occlude more distal portions of cortical vessels in order to achieve more focal ischemia targeting a smaller region of cortex. The primary disadvantages of pMCAO are that the surgical procedure is somewhat invasive as a small craniotomy is required to access MCA, though this results in minimal tissue damage. The primary advantages of this model, however, are: the site of occlusion is well defined, the degree of blood flow reduction is consistent, functional and neurological impairment occurs rapidly, infarct size is consistent, and the high rate of survival allows for long-term chronic assessment.

Permanent Cerebral Vessel Occlusion via Double Ligature and Transection

We describe a highly reproducible method for the permanent occlusion of a rodent major cerebral blood vessel. This technique can be accomplished with very little peripheral damage, minimal blood loss, a high rate of long-term survival, and consistent infarct volume commensurate with the human clinical population.

Stroke is a leading cause of death, disability, and socioeconomic loss worldwide. The majority of all strokes result from an interruption in blood flow ...

Uncontrolled Hemorrhagic Shock Modeled via Liver Laceration in Mice with Real Time Hemodynamic Monitoring

Uncontrolled hemorrhage is an important cause of preventable deaths among trauma patients. We have developed a murine model of uncontrolled hemorrhage via a liver laceration that results in consistent blood loss, hemodynamic alterations, and survival.
Mice undergo a standardized resection of the left-middle lobe of the liver. They are allowed to bleed without mechanical intervention. Hemostatic agents can be administered as pre-treatment or rescue therapy depending on the interest of the investigator. During the time of hemorrhage, real-time hemodynamic monitoring via a left femoral arterial line is performed. Mice are then sacrificed, blood loss is quantified, blood is collected for further analysis, and organs are harvested for analysis of injury. Experimental design is described to allow for simultaneous testing of multiple animals.
Liver hemorrhage as a model of uncontrolled hemorrhage exists in the literature, primarily in rat and porcine models. Some of these models utilize hemodynamic monitoring or quantify blood loss but lack consistency. The present model incorporates quantification of blood loss, real-time hemodynamic monitoring in a murine model that offers the advantage of using transgenic lines and a high-throughput mechanism to further investigate the pathophysiologic mechanisms in uncontrolled hemorrhage.

Uncontrolled hemorrhage, an important cause of mortality among trauma patients, can be modeled using a standard liver laceration in a murine model. This model results in consistent blood loss, survival, and allows for testing hemostatic agents. This article provides the step-by-step process to perform this valuable model.

Uncontrolled hemorrhage is an important cause of preventable deaths among trauma patients. We have developed a murine model of uncontrolled hemorrhage via ...

Balloon-based Injury to Induce Myointimal Hyperplasia in the Mouse Abdominal Aorta

The use of animal models is essential for a better understanding of MH, one major cause for arterial stenosis.In this article, we demonstrate a murine balloon denudation model, which is comparable with established vessel injury models in large animals. The aorta denudation model with balloon catheters mimics the clinical setting and leads to comparable pathobiological and physiological changes. Briefly, after performing a horizontal incision in the aorta abdominalis, a balloon catheter will be inserted into the vessel, inflated, and introduced retrogradely. Inflation of the balloon will lead to intima injury and overdistension of the vessel. After removing the catheter, the aortic incision will be closed with single stiches. The model shown in this article is reproducible, easy to perform, and can be established quickly and reliably. It is especially suitable for evaluating expensive experimental therapeutic agents, which can be applied in an economical fashion. By using different knockout-mouse strains, the impact of different genes on MH development can be assessed.

This article demonstrates a murine model to study the development of myointimal hyperplasia (MH) after aortic balloon injury.

The use of animal models is essential for a better understanding of MH, one major cause for arterial stenosis.In this article, we demonstrate a murine ...

Complete and Partial Aortic Occlusion for the Treatment of Hemorrhagic Shock in Swine

Hemorrhage remains the leading cause of preventable deaths in trauma. Endovascular management of non-compressible torso hemorrhage has been at the forefront of trauma care in recent years. Since complete aortic occlusion presents serious concerns, the concept of partial aortic occlusion has gained a growing attention. Here, we present a large animal model of hemorrhagic shock to investigate the effects of a novel partial aortic balloon occlusion catheter and compare it with a catheter that works on the principles of complete aortic occlusion. Swine are anesthetized and instrumented in order to conduct controlled fixed-volume hemorrhage, and hemodynamic and physiological parameters are monitored. Following hemorrhage, aortic balloon occlusion catheters are inserted and inflated in the supraceliac aorta for 60 min, during which the animals receive whole-blood resuscitation as 20% of the total blood volume (TBV). Following balloon deflation, the animals are monitored in a critical care setting for 4 h, during which they receive fluid resuscitation and vasopressors as needed. The partial aortic balloon occlusion demonstrated improved distal mean arterial pressures (MAPs) during the balloon inflation, decreased markers of ischemia, and decreased fluid resuscitation and vasopressor use. As swine physiology and homeostatic responses following hemorrhage have been well-documented and are like those in humans, a swine hemorrhagic shock model can be used to test various treatment strategies. In addition to treating hemorrhage, aortic balloon occlusion catheters have become popular for their role in cardiac arrest, cardiac and vascular surgery, and other high-risk elective surgical procedures.

Here, we present a protocol demonstrating a hemorrhagic shock model in swine that uses aortic occlusion as a bridge to definitive care in trauma. This model has application in testing a wide range of surgical and pharmacological therapeutic strategies.

Hemorrhage remains the leading cause of preventable deaths in trauma. Endovascular management of non-compressible torso hemorrhage has been at the ...

Standardized Hemorrhagic Shock Induction Guided by Cerebral Oximetry and Extended Hemodynamic Monitoring in Pigs

Hemorrhagic shock ranks among the main reasons for severe injury-related death. The loss of circulatory volume and oxygen carriers can lead to an insufficient oxygen supply and irreversible organ failure. The brain exerts only limited compensation capacities and is particularly at high risk of severe hypoxic damage.This article demonstrates the reproducible induction of life-threatening hemorrhagic shock in a porcine model by means of calculated blood withdrawal. We titrate shock induction guided by near-infrared spectroscopy&#160;and extended hemodynamic monitoring to display systemic circulatory failure, as well as cerebral microcirculatory oxygen depletion. In comparison to similar models that primarily focus on predefined removal volumes for shock induction, this approach highlights a titration by means of the resulting failure of macro- and microcirculation.

Hemorrhagic shock is a severe complication in seriously injured patients, which leads to life-threatening oxygen undersupply. We present a standardized method to induce hemorrhagic shock via blood withdrawal in pigs that is guided by hemodynamics and microcirculatory cerebral oxygenation.

Hemorrhagic shock ranks among the main reasons for severe injury-related death. The loss of circulatory volume and oxygen carriers can lead to an ...

The Intra-Aortic Balloon Pump

Cardiogenic shock remains one of the most challenging clinical syndromes in modern medicine. Mechanical support is being increasingly used in the management of cardiogenic shock. Intra-aortic balloon pump (IABP) is one of the earliest and most widely used types of mechanical circulatory support. The device acts by external counterpulsation and uses systolic unloading and diastolic augmentation of aortic pressure to improve hemodynamics. Although IABP provides less hemodynamic support when compared with newer mechanical circulatory support devices, it can still be the mechanical support device of choice in appropriate situations because of its relative simplicity of insertion and removal, need for smaller size vascular access and better safety profile. In this review, we discuss the equipment, procedural and technical aspects, hemodynamic effects, indications, evidence, current status and recent advances in the use of IABP in cardiogenic shock.

We describe the steps for the percutaneous implantation of the intra-aortic balloon pump (IABP), a mechanical circulatory support device. It acts by counterpulsation, inflating at the onset of diastole, augmenting diastolic aortic pressure and improving coronary blood flow and systemic perfusion, and deflating before systole, reducing left ventricular afterload.

Cardiogenic shock remains one of the most challenging clinical syndromes in modern medicine. Mechanical support is being increasingly used in the ...

Description of a Swine Infant Model of Volume-Controlled Hemorrhagic Shock

Hemorrhagic shock is a leading cause of morbidity and mortality in pediatric patients. Interpretation of the clinical indicators validated in adults to guide resuscitation and comparison between different therapies is difficult in children due to the inherent heterogeneity of this population. As a result, compared to adults, appropriate management of pediatric hemorrhagic shock is still not well established. In addition, the scarcity of pediatric patients with hemorrhagic shock precludes the development of clinically relevant studies. For this reason, an experimental pediatric animal model is necessary to study the effects of hemorrhage in children as well as their response to different therapies. We present an infant animal model of volume-controlled hemorrhagic shock in anesthetized young pigs. Hemorrhage is induced by withdrawing a previously calculated blood volume, and the pig is subsequently monitored and resuscitated with different therapies. Here, we describe a precise and highly reproducible model of hemorrhagic shock in immature swine. The model yields hemodynamic data that characterizes compensatory mechanisms that are activated in response to severe hemorrhage.

This article aims to provide researchers with a detailed and accessible guide to set up an infant porcine model of hemorrhagic shock.

Hemorrhagic shock is a leading cause of morbidity and mortality in pediatric patients. Interpretation of the clinical indicators validated in adults to ...

Vascular Cannulation with Hemorrhagic Shock Induction in Rats

Developing a Clinically Relevant Hemorrhagic Shock Model in Rats

Over the recent decades, the development of animal models allowed us to better understand various pathologies and identify new treatments. Hemorrhagic shock, i.e., organ failure due to rapid loss of a large volume of blood, is associated with a highly complex pathophysiology involving several pathways. Numerous existing animal models of hemorrhagic shock strive to replicate what happens in humans, but these models have limits in terms of clinical relevance, reproducibility, or standardization. The aim of this study was to refine these models to develop a new model of hemorrhagic shock. Briefly, hemorrhagic shock was induced in male Wistar Han rats (11-13 weeks old) by a controlled exsanguination responsible for a drop in the mean arterial pressure. The next phase of 75 min was to maintain a low mean arterial blood pressure, between 32 mmHg and 38 mmHg, to trigger the pathophysiological pathways of hemorrhagic shock. The final phase of the protocol mimicked patient care with an administration of intravenous fluids, Ringer Lactate solution, to elevate the blood pressure. Lactate and behavioral scores were assessed 16 h after the protocol started, while hemodynamics parameters and plasmatic markers were evaluated 24 h after injury. Twenty-four hours post-hemorrhagic shock induction, the mean arterial and diastolic blood pressure were decreased in the hemorrhagic shock group (p&#160;&#60; 0.05). Heart rate and systolic blood pressure remained unchanged. All organ damage markers were increased with the hemorrhagic shock (p &#60; 0.05). The lactatemia and behavioral scores were increased compared to the sham group (p &#60; 0.05). In conclusion, we demonstrated that the protocol described here is a relevant model of hemorrhagic shock that can be used in subsequent studies, particularly to evaluate the therapeutic potential of new molecules.

Author Spotlight: Developing Innovative Therapeutic Strategies for Hemorrhagic Shock Research

Hemorrhagic shock kills 1.9 million people worldwide every year. Small animals are frequently used as hemorrhagic shock models but are associated with issues of standardization, reproducibility, and clinical significance, thus limiting their relevance. This article describes developing a new clinically relevant hemorrhagic shock model in rats.

Over the recent decades, the development of animal models allowed us to better understand various pathologies and identify new treatments. Hemorrhagic ...

Closed-Chest Technique for Murine Myocardial Ischemia-Reperfusion Injury

Remotely Triggered LAD Occlusion Using a Balloon Catheter in Spontaneously Breathing Mice

Acute myocardial infarction (AMI) is a prevalent and high-mortality cardiovascular condition. Despite advancements in revascularization strategies for AMI, it frequently leads to myocardial ischemia-reperfusion injury (IRI), amplifying cardiac damage. Murine models serve as vital tools for investigating both acute injury and chronic myocardial remodeling in vivo. This study presents a unique closed-chest technique for remotely inducing myocardial IRI in mice, enabling the investigation of the very early phase of occlusion and reperfusion using in-vivo imaging such as MRI or PET. The protocol utilizes a remote occlusion method, allowing precise control over ischemia initiation after chest closure. It reduces surgical trauma, enables spontaneous breathing, and enhances experimental consistency. What sets this technique apart is its potential for simultaneous noninvasive imaging, including ultrasound and magnetic resonance imaging (MRI), during occlusion and reperfusion events. It offers a unique opportunity to analyze tissue responses in almost real-time, providing critical insights into processes during ischemia and reperfusion. Extensive systematic testing of this innovative approach was conducted, measuring cardiac necrosis markers for infarction, assessing the area at risk using contrast-enhanced MRI, and staining infarcts at the scar maturation stage. Through these investigations, emphasis was placed on the value of the proposed tool in advancing research approaches to myocardial ischemia-reperfusion injury and accelerating the development of targeted interventions. Preliminary findings demonstrating the feasibility of combining the proposed innovative experimental protocol with noninvasive imaging techniques are presented herein. These initial results highlight the benefit of utilizing the purpose-built animal cradle to remotely induce myocardial ischemia while simultaneously conducting MRI scans.

Author Spotlight: A Novel Standardized Technique for Real-Time Biomedical Imaging of Acute Myocardial Injury

This article presents a unique closed-chest technique for inducing myocardial ischemia-reperfusion injury (IRI) in mice. The presented method allows mice to breathe spontaneously while remotely inducing myocardial ischemia. This provides access to the animal for studying the dynamic processes of ischemia and reperfusion in situ and in real-time via noninvasive imaging.

Acute myocardial infarction (AMI) is a prevalent and high-mortality cardiovascular condition. Despite advancements in revascularization strategies for ...