A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells
1.4K Views
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09:10 min
•
June 23rd, 2022
DOI :
June 23rd, 2022
•Benson Koh1,2, Kian Leong Tan3, Hong Hao Chan4, Qi Hao Daniel Looi2, Moon Nian Lim5, Chee Wun How4, Jia Xian Law1, Jhi Biau Foo6,7
1Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, 2My CytoHealth Sdn. Bhd., 3School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, 4School of Pharmacy, Monash University Malaysia, 5Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, 6School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, 7Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University
Chapters in this video
0:05
Introduction
1:09
Small Extracellular Vesicle Isolation from hUC‐MSCs
2:48
Characterization of hUC‐MSC‐sEVs
7:14
Results: Nanoparticle Tracking Analysis, Biomarker Expression, and Morphology of Single hUC‐MSC‐sEVs
8:39
Conclusion
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