Differential Centrifugation for Isolation of Exosomes and Microvesicles
2:25
Detection of Particle Numbers and Size Distribution of Extracellular Vesicles
3:29
Labeling of Extracellular Vesicles Derived from Mesenchymal Stem Cells
4:50
Systemic Injection of Extracellular Vesicles Derived from Mesenchymal Stem Cells
5:24
Results: Characterization of Extracellular Vesicles Derived from Mesenchymal Stem Cells
6:19
Conclusion
Transcript
This procedure addressed the need for simple and reliable mesenchymal stem cell-derived extracellular vesicle collection, and of application in translation of research. Collection of extracellular vesicles by differential centrifugation is simple,
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The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this article.