Results: Determination of Storage Temperature for Amniotic Membrane Extract
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Conclusion
Transcript
This method aims to demonstrate a straightforward technique for protein extraction, useful for the obtaintion of bountiful proteins and other factors. This technique could be used to extract other factors and proteins present in the amniotic membr
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The present protocol describes the extraction of lumican from the amniotic membrane (AM) and their storing conditions as AM extract (AME) at -20 °C, 4 °C, and room temperature (RT) for 6, 12, 20, and 32 days to quantify its proteins and lumican concentration.