Results: Bacterial Co-Expression of Differentially Tagged Proteins
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Conclusion
Transcript
This method allows to study formations of protein complexes that require co-expression for efficient test samples, such as the formation of heterodimers. Main end potential of this method is exploiting the co-expression of differentially-tagged st
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Here, we describe a method for the bacterial co-expression of differentially tagged proteins using a set of compatible vectors, followed by the conventional pulldown techniques to study protein complexes that cannot assemble in vitro.