JoVE Logo
Faculty Resource Center

Sign In

Intravital Imaging of Fluorescent Protein Expression in Mice with a Closed-Skull Traumatic Brain Injury and Cranial Window Using a Two-Photon Microscope

DOI :

10.3791/64701-v

April 21st, 2023

April 21st, 2023

631 Views

1Department of Neurology, University of Massachusetts Chan Medical School, 2Department of Neurosurgery, The First Affiliated Hospital of Chongqing Medical University, 3Department of Neurobiology, University of Massachusetts Chan Medical School

This study demonstrates delivery of a repetitive traumatic brain injury to mice and simultaneous implantation of a cranial window for subsequent intravital imaging of a neuron-expressed EGFP using two-photon microscopy.

-- Views

Related Videos

article

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

article

Extinction Training During the Reconsolidation Window Prevents Recovery of Fear

article

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging

article

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury

article

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

article

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

article

Author Spotlight: Advancements in Adult Zebrafish Brain Research

article

Author Spotlight: Imaging Pericytes Post-Subarachnoid Hemorrhaging in Rodents

article

Author Spotlight: Advancing Traumatic Brain Injury Research — A Closed-Head Model for Accurate Replication and Rapid Assessment

article

Two-Photon Imaging of Zebrafish Forebrain

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved