JoVE Logo
Faculty Resource Center

Sign In

Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran

DOI :

10.3791/64710-v

5:14 min

November 18th, 2022

November 18th, 2022

2,909 Views

1Nutrition and Microbiome Laboratory, Institut du cancer de Montréal, Centre de recherche du Centre hospitalier de l’Université de Montréal (CRCHUM), 2Digestive Surgery Service, Centre hospitalier de l’Université de Montréal (CHUM), Faculty of Medicine, Université de Montréal, 3Department of Medicine, Faculty of Medicine, Université de Montréal

In the present study, fluorescein-isothiocyanate-labeled (FITC) dextran is administered to mice via oral gavage to evaluate intestinal permeability both in vivo and in plasma and fecal samples. As gut barrier function is affected in many disease processes, this direct and quantitative assay can be used in diverse research areas.

Tags

Assessment

-- Views

Related Videos

article

Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes

article

Murine Colitis Modeling using Dextran Sulfate Sodium (DSS)

article

Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran

article

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

article

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

article

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

article

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

article

High-throughput Measurement of Gut Transit Time Using Larval Zebrafish

article

Assessment of Zebrafish Lens Nucleus Localization and Sutural Integrity

article

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved