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An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance Video (Video) | JoVE

DOI :

10.3791/60790-v

December 3rd, 2020

December 3rd, 2020

5,402 Views

1Medical Clinic 1, University of Erlangen-Nuremberg, University Hospital of Erlangen, 2Norwich Medical School, University of East Anglia, Norwich Research Park

Taking advantage of intravital microscopy, the method presented here enables real-time visualization of intestinal epithelial cell shedding in living animals. Therefore, topically stained intestinal mucosa (acriflavine and rhodamineB-dextran) of anesthetized mice is imaged up to single-cell resolution using confocal microscopy.

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