JoVE Logo
Faculty Resource Center

Sign In

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

DOI :

10.3791/57038-v

February 26th, 2018

February 26th, 2018

23,009 Views

1Institute of Neurology (Edinger Institute), Goethe University Hospital, 2Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology, Goethe University Hospital

Here we present a mouse brain vascular permeability assay using intraperitoneal injection of fluorescent tracers followed by perfusion that is applicable to animal models of blood-brain barrier dysfunction. One hemi-brain is used for assessing permeability quantitatively and the other for tracer visualization/immunostaining. The procedure takes 5 - 6 h for 10 mice.

Tags

In Vivo Blood brain Barrier Permeability Assay

-- Views

Related Videos

article

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

article

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

article

An Assay for Permeability of the Zebrafish Embryonic Neuroepithelium

article

Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease

article

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

article

A Benchtop Approach to the Location Specific Blood Brain Barrier Opening using Focused Ultrasound in a Rat Model

article

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice

article

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

article

Applications of TEER Detection to Assess Cell Barrier Integrity (Video) | JoVE

article

A Personalized Approach Towards Investigating Alzheimer's Disease Using an In Vitro Blood-Brain Barrier Model (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved