Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
2.6K Views
•
07:14 min
•
June 16th, 2023
DOI :
June 16th, 2023
•Pavan S. Krishnan1,2, Fernando T. Zamuner1,3, Carolyn M. Jenks1, Johnny Y. Xie4, Lisa Zhang5, Mohammed Lehar1, Neal S. Fedarko6, Mariana Brait1,3, John P. Carey1
1Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery, The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory, Johns Hopkins University School of Medicine
Chapters in this video
0:04
Introduction
1:03
Collection, Preparation, and Extraction of Plasma Sample
3:28
ELISA Plate Preparation (Blocking)
4:16
Enzyme-Linked Immunosorbent Assay (ELISA)
6:06
Results: Effect of Blocking on CGRP Quantification
6:29
Conclusion
Related Videos
09:23
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
6.2K Views
08:29
Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
82 Views
07:30
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
9.2K Views
11:03
Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets
7.2K Views
10:12
A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
16.7K Views
07:28
Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing
4.0K Views
08:39
Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
2.3K Views
11:17
Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
16.4K Views
03:57
Generation of Hypoparathyroid Rats via Carbon-Nanoparticle-Assisted Parathyroidectomy
1.8K Views
01:33
Detection of Human Islet Autoantibodies Using an Electrochemiluminescence Assay
54 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved