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Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures

DOI :

10.3791/65102-v

8:42 min

May 26th, 2023

May 26th, 2023

845 Views

1PulmoScience Laboratory, Department of Pulmonology, Leiden University Medical Center

Presented here is a reproducible, affordable, and robust method for the isolation and expansion of primary bronchial epithelial cells for long-term biobanking and the generation of differentiated epithelial cells by culture at the air-liquid interface.

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Isolating Bronchial Epithelial Cells

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