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An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

DOI :

10.3791/53256-v

October 29th, 2015

October 29th, 2015

11,675 Views

1Department of Rheumatology, Laboratory for Molecular Immunology and Inflammation, Ghent University Hospital, 2VIB Inflammation Research Center, Ghent University

Here we present an adapted protocol that can be used to generate a large number of murine invariant natural killer T cells from mouse spleen. The protocol outlines an approach by which splenic iNKT cells can be enriched for, isolated and expanded in vitro using a limited number of animals and reagents.

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Optimized Method

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