Our research aims to establish a mouse model of Candida albicans associated catheter-related infection, in which biofilm forms on the catheter. This model is helpful for correlating with the clinical CRI and the interaction between Candida albicans and host. The existing animal models of Candida albicans, such as the oropharyngeal candidiasis model, vaginal candidiasis model, and invasive systemic infection model caused by candidiasis cannot correlate well with the clinical CRI.
To improve Candida albicans detection in catheter infections, our protocol employed a stain with enhanced green fluorescein protein. This allowed for clear visualization of Candida albicans colony and biofilms on catheters in mice, and in timely detection.
