Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

2.9K Views

11:04 min

June 23rd, 2023

DOI :

10.3791/65463-v

June 23rd, 2023


Transcript

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Electroporation

Chapters in this video

0:00

Introduction

2:04

Preparing Limosilactobacillus reuteri DSM20016 Electrocompetent Cells for Electroporation

5:27

Measurement of the Acid‐Resistant Fluorescent Reporter Protein mCherry2

7:03

Confirmation of Plasmid Uptake via Colony PCR

8:45

Mini‐Prep Protocol for Limosilactobacillus reuteri and Confirmation of Plasmid Presence by PCR

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