Methods Article

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

2.3K Views

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06:45 min

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June 2nd, 2023

DOI :

10.3791/65631-v

June 2nd, 2023

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Mingqing Tang¹^,², Hongjun Lin¹, Chengjia Hu¹, Hui Yan¹^,²

¹Medicine School, Huaqiao University, ²Engineering Research Centre of Molecular Medicine of Ministry of Education

Transcript

Summary

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Affinity Purification

Plasminogen Activation

Thrombolytic Agents

Chromatography

Structural Information

Purification Protocol

Enzyme Characterization

Purification Efficiency

SFE Purity

Chapters in this video

0:00

Introduction

0:59

Protein Isolation from Sipunculus nudus

2:19

Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts

4:49

Fibrinolytic Activity of the Fibrinolytic Enzyme Purified from Sipunculus nudus

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