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Protein Complex Affinity Capture from Cryomilled Mammalian Cells

DOI :

10.3791/54518-v

10:37 min

December 9th, 2016

December 9th, 2016

14,116 Views

1Laboratory of Cellular and Structural Biology, The Rockefeller University, 2Institute for Systems Genetics, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine

Here we describe protocols to disrupt mammalian cells by solid-state milling at a cryogenic temperature, produce a cell extract from the resulting cell powder, and isolate protein complexes of interest by affinity capture upon antibody-coupled micron-scale paramagnetic beads.

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Protein Complex Affinity Capture

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