High-Throughput Bioprinting Method for Modeling Vascular Permeability in Standard Six-well Plates with Size and Pattern Flexibility

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07:41 min

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August 16th, 2024

DOI :

10.3791/66676-v

August 16th, 2024

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Ashfaq Ahmad¹^,², Mst Zobaida Akter¹^,², Seo-Yeon Kim¹^,², Yeong-Jin Choi³^,⁴, Hee-Gyeong Yi¹^,²

¹Department of Convergence Biosystems Engineering, College of Agriculture and Life Sciences (CALS), Chonnam National University, ²Interdisciplinary Program in IT-Bio Convergence System, Chonnam National University, ³Advanced Bio and Healthcare Materials Research Division, Korea Institute of Materials Science (KIMS), ⁴Advanced Materials Engineering, Korea National University of Science and Technology (UST)

Summary

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High throughput Bioprinting

Vascular Permeability

Preclinical Testing

Multi material Bioprinting

In Vitro Models

Computer controlled 3D Printing

Human Blood Vessel Models

Endothelial Dysfunction

Fabrication Techniques

Chapters in this video

0:00

Introduction

1:12

Programming and Preparation of a 3D Bioprinter to Produce Vessels‐on‐a‐Plate (VOP) for Cell Culture

2:50

Fabrication of Vessels‐on‐a‐Plate (VOP) Platform Using a 3D Bioprinter to Facilitate Cell Culture

3:52

Hydrogel Preparation and Channel Embedding of a Vessels‐on‐a‐Plate (VOP) Platform for the Culture of Human Umbilical Vein Endothelial Cells (HUVEC)

4:51

Human Umbilical Vein Endothelial Cell (HUVEC) Endothelization of a Vessels‐on‐a‐Plate (VOP) Platform

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