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Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

DOI :

10.3791/690-v

March 28th, 2008

March 28th, 2008

18,845 Views

1Department of Cell and Tissue Biology, University of California, San Francisco - UCSF

Proteins bind to filamentous actin (F-actin) through distinct actin binding modules. In this video we demonstrate the procedure of actin co-sedimentation, which is an in vitro assay routinely used to analyze proteins or specific domains that bind F-actin.

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Actin Co sedimentation Assay

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