Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

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07:53 min

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March 28th, 2008

DOI :

March 28th, 2008

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Jyoti Srivastava¹, Diane Barber¹

¹Department of Cell and Tissue Biology, University of California, San Francisco - UCSF

Summary

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Actin Co sedimentation Assay

Protein Binding

Actin Cytoskeleton

Cellular Processes

Dynamic Structure

Protein Analysis

Ultracentrifugation

Protein Co sedimenting

Actin Binding Affinities

Competition Assays

Chapters in this video

0:00

Title

0:42

Introduction

1:44

Preparation of Actin Filaments

2:49

Preparation of Protein and Binding of Actin

4:26

Running the Gel

6:58

Conclusion

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