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Reconstitution of Actin-Based Motility with Commercially Available Proteins

DOI :

10.3791/64261-v

8:40 min

October 28th, 2022

October 28th, 2022

1,120 Views

1Laboratoire de physique de l’Ecole Normale Supérieure, ENS, Université PSL, CNRS, Sorbonne Université, Université Paris Cité

This protocol describes how to produce actin comets on the surfaces of beads using commercially available protein ingredients. Such systems mimic the protrusive structures found in cells, and can be used to examine physiological mechanisms of force production in a simplified way.

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Actin based Motility

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