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Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates

DOI :

10.3791/820-v

November 5th, 2008

November 5th, 2008

14,624 Views

1Department of Pharmacology and Experimental Therapeutics, Tufts University

Primary dissociated midbrain dopamine cell cultures allow for the study of presynaptic characteristics of dopamine neurons. They can be used to monitor real-time dopamine release kinetics and protein/mRNA levels of regulators of dopamine exocytosis. Here, we show you how to generate these cultures from rodent neonates.

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Primary Cell Cultures

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