JoVE Logo

Sign In

Studying Pre-formed Fibril Induced α-Synuclein Accumulation in Primary Embryonic Mouse Midbrain Dopamine Neurons

10.4K Views

10:03 min

August 16th, 2020

DOI :

10.3791/61118-v

August 16th, 2020


Explore More Videos

Alpha synuclein Accumulation

Chapters in this video

0:04

Introduction

1:11

Primary Embryonic Midbrain Culture Plate Preparation

1:53

Primary E13.5 Embryonic Midbrain Culture Setup

4:32

Seeding with Pre-Formed Fibrils (PFFs) and Semi-automated Immunofluorescence Imaging

6:21

High-Content Image Analysis

7:42

Results: Representative Midbrain Culture Characterization and Analysis

9:13

Conclusion

Related Videos

article

11:58

Primary Culture of Mouse Dopaminergic Neurons

37.6K Views

article

12:01

Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein

10.6K Views

article

08:33

Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector

13.2K Views

article

09:16

Exogenous Administration of Microsomes-associated Alpha-synuclein Aggregates to Primary Neurons As a Powerful Cell Model of Fibrils Formation

7.5K Views

article

09:44

Generation of Alpha-Synuclein Preformed Fibrils from Monomers and Use In Vivo

21.2K Views

article

06:54

Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

6.2K Views

article

14:45

Analyzing the Parkinson's Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein

5.8K Views

article

04:49

Technique for Intranasal Administration of α-Synuclein Aggregates

348 Views

article

01:19

Induction of Neuroinflammation in Mice via Injection of Alpha-Synuclein Fibrils

27 Views

article

01:29

Studying Alpha-Synuclein Accumulation in Primary Embryonic Mouse Dopamine Neurons

36 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved