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The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

DOI :

10.3791/51040-v

March 11th, 2014

March 11th, 2014

18,115 Views

1Department of Biomedical Engineering, Washington University in St. Louis

The cut-open Vaseline gap approach is used to obtain low noise recordings of ionic and gating currents from voltage-dependent ion channels expressed in Xenopus oocytes with high resolution of fast channel kinetics. With minor modification, voltage clamp fluorometry can be coupled to the cut-open oocyte protocol.

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Xenopus Oocyte

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