The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry Video (Video) | JoVE
March 11th, 2014
•The cut-open Vaseline gap approach is used to obtain low noise recordings of ionic and gating currents from voltage-dependent ion channels expressed in Xenopus oocytes with high resolution of fast channel kinetics. With minor modification, voltage clamp fluorometry can be coupled to the cut-open oocyte protocol.
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